1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GALNT2 蛋白, Human (HEK293, His)

GALNT2 蛋白, Human (HEK293, His)

目录号: HY-P76355
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GALNT2 蛋白通过将 N-乙酰基-D-半乳糖胺转移至蛋白受体来催化 O-连接寡糖生物合成的初始步骤。GALNT2 蛋白, Human (HEK293, His) 是重组的 GALNT2 蛋白,由 HEK293 表达,带有 N-His 标签。GALNT2 蛋白, Human (HEK293, His) 全长 520 个氨基酸,分子量分别为 63 KDa。

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10 μg ¥1500 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The GALNT2 protein catalyzes the initial steps in O-linked oligosaccharide biosynthesis by transferring N-acetyl-D-galactosamine to a protein receptor. GALNT2 Protein, Human (HEK293, His) is the recombinant human-derived GALNT2 protein, expressed by HEK293 , with N-His labeled tag. The total length of GALNT2 Protein, Human (HEK293, His) is 520 a.a., with molecular weight of 63 KDa, respectively.

研究背景

GALNT2 蛋白是 O-连接寡糖生物合成中的关键酶,通过将 N-乙酰基-D-半乳糖胺残基转移到蛋白受体上的丝氨酸或苏氨酸残基来启动该过程。这种酶活性表现出广泛的底物谱,包括 EA2、Muc5AC、Muc1a 和 Muc1b 等肽。GALNT2 参与关键蛋白质的 O 连接糖基化,包括免疫球蛋白 A1 (IgA1) 铰链区、APOC-III、ANGPTL3 和 PLTP。此外,它还在高密度脂蛋白胆固醇 (HDL-C) 代谢的调节中发挥作用,有助于控制脂质稳态的复杂过程。

生物活性

Measured by its ability to transfer GalNAc from UDP-GalNAc to EA2 that incubate at 37°C for 20 min. The specific activity is 379.51-404.75 pmol/min/μg.

种属

Human

表达系统

HEK293

标签

N-10*His

蛋白编号

Q10471-1/NP_004472.1 (K52-Q571)

基因 ID
蛋白结构
N-term
His
GALNT2 (K52-Q571)
Accession # Q10471/NP_004472.1
C-term
同用名
Polypeptide N-acetylgalactosaminyltransferase 2; GalNAc-T2
氨基酸序列

KKKDLHHSNGEEKAQSMETLPPGKVRWPDFNQEAYVGGTMVRSGQDPYARNKFNQVESDKLRMDRAIPDTRHDQCQRKQWRVDLPATSVVITFHNEARSALLRTVVSVLKKSPPHLIKEIILVDDYSNDPEDGALLGKIEKVRVLRNDRREGLMRSRVRGADAAQAKVLTFLDSHCECNEHWLEPLLERVAEDRTRVVSPIIDVINMDNFQYVGASADLKGGFDWNLVFKWDYMTPEQRRSRQGNPVAPIKTPMIAGGLFVMDKFYFEELGKYDMMMDVWGGENLEISFRVWQCGGSLEIIPCSRVGHVFRKQHPYTFPGGSGTVFARNTRRAAEVWMDEYKNFYYAAVPSARNVPYGNIQSRLELRKKLSCKPFKWYLENVYPELRVPDHQDIAFGALQQGTNCLDTLGHFADGVVGVYECHNAGGNQEWALTKEKSVKHMDLCLTVVDRAPGSLIKLQGCRENDSRQKWEQIEGNSKLRHVGSNLCLDSRTAKSGGLSVEVCGPALSQQWKFTLNLQQ

分子量

Approximately 63 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 25 mM Tris, 150 mM NaCl, pH 7.5 or 25 mM Tris-HCL, 150 mM NaCl, pH 7.5, 8% trehalose.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GALNT2 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GALNT2 Protein, Human (HEK293, His)
目录号:
HY-P76355
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