GAS6 蛋白, Human (HEK293, Fc)

GAS6 Protein is a secreted protein located outside the cell membrane. GAS6 Protein is a ligand for tyrosine protein kinase receptors AXL, TYRO3, and MER, and its signal transduction is related to cell growth and survival, cell adhesion, and cell migration. GAS6 is associated with tumor cell growth, metastasis, invasion, epithelial mesenchymal transition, angiogenesis, drug resistance, immune regulation, and stem cell maintenance after binding to AXL. GAS6 Protein, Human (HEK293, Fc) is a recombinant GAS6 protein with an Fc tag, expressed by HEK293^[1][2].

GAS6 Protein 由生长停滞特异性基因 6 (Gas6) 编码，属于维生素 K 依赖性蛋白家族^[2]。
GAS6 Protein-AXL 可以调节 PI3K/AKT/mTOR、JAK/STAT、NF-κB 和 RAS/RAF/MEK/ERK 等信号通路，在肿瘤细胞存活、抗细胞凋亡信号传导、有丝分裂发生、迁移、侵袭、耐药、血管生成和肿瘤-宿主关系中发挥作用^[2]。
GAS6 Protein-AXL 可以促进内皮细胞存活，调节人类自然杀伤细胞发育过程中的最佳细胞因子信号转导、肝脏再生、促性腺激素释放激素神经元存活和迁移、血小板活化或血栓反应；也可以引起肌动蛋白重塑，从而驱动膜皱褶、巨细胞吞噬和癌细胞侵袭^[1][3]。
GAS6 Protein 信号传导通过抑制炎症、氧化应激、线粒体损伤和 ER 应激在脓毒症心脏中发挥有利作用，其中 NLRP3 是介导 GAS6 信号转导的主要分子^[4]。
GAS6 Protein 通过将病毒颗粒包膜磷脂酰丝氨酸桥接到靶细胞上的 TAM 受体酪氨酸激酶 AxI，从而介导病毒与靶细胞的结合，促进病毒进入^[5]。
GAS6 Protein 与肾炎、肿瘤、病毒感染、心血管疾病等病理过程密切相关^[1][4][5]。

GAS6 Protein (400 ng/mL, 10 min) 可以在人胶质母细胞瘤 LN229 细胞中触发膜褶皱，表现为外围皱褶 (PR) 和环状背侧皱褶 (CDR) 的形成^[1]。
GAS6 Protein (25-100 ng/mL, 3 h) 可以抑制炎症因子和 NLRP3、ASC 蛋白的表达，从而减轻 LPS 诱导的 HL-1 细胞炎症^[4]。

GAS6 全身敲除 (GAS6 KO) 小鼠中 GAS6 Protein 表达缺失，会导致脓毒症小鼠体内脓毒性心肌损伤加重^[4]。
GAS6 Protein (2 μg; 每天 1 次; 10 天; 腹腔注射) 在 GAS6^–/– 贫血小鼠中诱导红细胞生成效应并缓解贫血^[6]。
GAS6 Protein (2 μg; 每天 1 次; 4 天; 注射) 在 GAS6^–/– 肾毒性肾炎小鼠中可以增加蛋白尿和每个肾小球的 PCNA 阳性细胞数，从而促进肾炎发展^[7]。

Immobilized Human AXL-His at 5 μg/mL (100 μl/well) can bind Human GAS6-Fc. The ED₅₀ of Human GAS6-Fc is ≤27 μg/mL.

Human

HEK293

C-hFc

Q14393-2 (A31-A678)

2621  [NCBI]

ALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA

110-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zdżalik-Bielecka D, et al. The GAS6-AXL signaling pathway triggers actin remodeling that drives membrane ruffling, macropinocytosis, and cancer-cell invasion. Proc Natl Acad Sci U S A. 2021 Jul 13;118(28):e2024596118.  [Content Brief]

  [2]. Zhu C, et al. AXL receptor tyrosine kinase as a promising anti-cancer approach: functions, molecular mechanisms and clinical applications. Mol Cancer. 2019 Nov 4;18(1):153.  [Content Brief]

  [3]. Park IK, et al. The Axl/Gas6 pathway is required for optimal cytokine signaling during human natural killer cell development. Blood. 2009 Mar 12;113(11):2470-7.  [Content Brief]

  [4]. Ji T, et al. GAS6 attenuates sepsis-induced cardiac dysfunction through NLRP3 inflammasome-dependent mechanism. Free Radic Biol Med. 2024 Jan;210:195-211.  [Content Brief]

  [5]. Morizono K, et al. The soluble serum protein Gas6 bridges virion envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry. Cell Host Microbe. 2011 Apr 21;9(4):286-98.  [Content Brief]

  [6]. Angelillo-Scherrer A, et al. Role of Gas6 in erythropoiesis and anemia in mice. J Clin Invest. 2008 Feb;118(2):583-96.  [Content Brief]

  [7]. Yanagita M, et al. Essential role of Gas6 for glomerular injury in nephrotoxic nephritis. J Clin Invest. 2002 Jul;110(2):239-46.  [Content Brief]