GCKR 蛋白, Human (P.pastoris, His)

The GCKR protein plays a critical regulatory role in glucokinase (GCK) by forming an inactive complex and recruiting GCK to the nucleus after meals. Its interaction with GCK is finely tuned by fructose metabolites; binding of GCKR to fructose-6-phosphate increases the affinity for GCK, whereas binding to fructose-1-phosphate decreases the affinity, thereby releasing inhibition. GCKR Protein, Human (P.pastoris, His) is the recombinant human-derived GCKR protein, expressed by P. pastoris , with N-His labeled tag.

GCKR 蛋白与葡萄糖激酶 (GCK) 形成无活性复合物，在该酶的调节中发挥着至关重要的作用。它的作用是促进 GCK 募集到细胞核，潜在地形成一个 GCK 库，可以在餐后快速释放到细胞质中。GCKR 和 GCK 之间的相互作用通过果糖代谢物进行微调，其中 GCKR 与果糖 6-磷酸结合后表现出对 GCK 的亲和力增加。相反，与 1-磷酸果糖结合的 GCKR 对 GCK 的亲和力显着降低，导致对 GCK 活性的抑制作用丧失。这种动态相互作用强调了 GCKR 调节 GCK 功能的复杂调节机制。此外，GCKR 与 GCK 特异性地以其果糖 6-磷酸结合形式相互作用，突出了参与 GCK 调节的分子相互作用的特异性。

Human

P. pastoris

N-His

Q14397 (M1-Q625)

2646  [NCBI]

MPGTKRFQHVIETPEPGKWELSGYEAAVPITEKSNPLTQDLDKADAENIVRLLGQCDAEIFQEEGQALSTYQRLYSESILTTMVQVAGKVQEVLKEPDGGLVVLSGGGTSGRMAFLMSVSFNQLMKGLGQKPLYTYLIAGGDRSVVASREGTEDSALHGIEELKKVAAGKKRVIVIGISVGLSAPFVAGQMDCCMNNTAVFLPVLVGFNPVSMARNDPIEDWSSTFRQVAERMQKMQEKQKAFVLNPAIGPEGLSGSSRMKGGSATKILLETLLLAAHKTVDQGIAASQRCLLEILRTFERAHQVTYSQSPKIATLMKSVSTSLEKKGHVYLVGWQTLGIIAIMDGVECIHTFGADFRDVRGFLIGDHSDMFNQKAELTNQGPQFTFSQEDFLTSILPSLTEIDTVVFIFTLDDNLTEVQTIVEQVKEKTNHIQALAHSTVGQTLPIPLKKLFPSIISITWPLLFFEYEGNFIQKFQRELSTKWVLNTVSTGAHVLLGKILQNHMLDLRISNSKLFWRALAMLQRFSGQSKARCIESLLRAIHFPQPLSDDIRAAPISCHVQVAHEKEQVIPIALLSLLFRCSITEAQAHLAAAPSVCEAVRSALAGPGQKRTADPLEILEPDVQ

Approximately 70.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.