GFRAL 蛋白, Human (Biotinylated, HEK293, His-Avi)

The GFRAL protein is a brainstem-restricted receptor for GDF15 that regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stress. GFRAL binds to its ligand GDF15, interacts with RET, and activates the MAPK and AKT signaling pathways. GFRAL Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived GFRAL protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of GFRAL Protein, Human (Biotinylated, HEK293, His-Avi) is 333 a.a., with molecular weight of 48-62 kDa.

GFRAL 蛋白是 GDF15 的脑干限制性受体，在调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力方面发挥着至关重要的作用。在与其配体 GDF15 结合后，GFRAL 与 RET 相互作用，并通过 MAPK 和 AKT 信号通路激活细胞信号传导。该受体通过其胞外结构域与 GDF15 和 RET 形成复合物，在 GDF15 结合后 RET 参与时特异性介导细胞信号传导。这种复杂的相互作用凸显了 GFRAL、GDF15 和 RET 在调节代谢挑战的生理反应中的连续步骤。

1.Immobilized Biotinylated Human GFRAL His at 0.5 μg/mL (100μL/Well) on the plate. Dose response curve for Human GFD15 hFc with the EC₅₀ of 7.9 ng/mL determined by ELISA.
2.Immobilized Human GDF15,His Tag at 1ug/ml (100μL/well)on the plate.Dose response curve for Biotinylated Human GFRAL,His Tag with the EC₅₀ of 0.08 μg/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

Q6UXV0 (S19-E351)

389400  [NCBI]

SQTNNCTYLREQCLRDANGCKHAWRVMEDACNDSDPGDPCKMRNSSYCNLSIQYLVESNFQFKECLCTDDFYCTVNKLLGKKCINKSDNVKEDKFKWNLTTRSHHGFKGMWSCLEVAEACVGDVVCNAQLASYLKACSANGNPCDLKQCQAAIRFFYQNIPFNIAQMLAFCDCAQSDIPCQQSKEALHSKTCAVNMVPPPTCLSVIRSCQNDELCRRHYRTFQSKCWQRVTRKCHEDENCISTLSKQDLTCSGSDDCKAAYIDILGTVLQVQCTCRTITQSEESLCKIFQHMLHRKSCFNYPTLSNVKGMALYTRKHANKITLTGFHSPFNGE

48-62 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.