1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. TGF-beta Superfamily Neurotrophic Factors
  4. GDNF family
  5. GFRAL
  6. GFRAL 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

GFRAL 蛋白, Mouse (Biotinylated, HEK293, His-Avi)

目录号: HY-P700728
产品使用指南

GFRAL 蛋白是一种脑干限制性受体,可调节食物摄入、能量消耗和体重,以应对代谢和毒素引起的压力。它与 GDF15 结合,与 RET 相互作用,并激活 MAPK 和 AKT 信号通路。GFRAL 充当 GDF15 的受体,并通过 RET 介导细胞信号传导,需要事先结合 GDF15。GFRAL 蛋白, Mouse (Biotinylated, HEK293, His-Avi) 是重组的 GFRAL 蛋白,由 HEK293 表达,带有 C-Avi, N-His 标签。

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     可免费申领三个不同产品的试用装。

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描述

GFRAL protein, a brainstem-restricted receptor, regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stresses. It binds with GDF15, interacts with RET, and activates MAPK- and AKT- signaling pathways. GFRAL acts as a receptor for GDF15 and mediates cellular signaling through RET, requiring prior GDF15 binding. GFRAL Protein, Mouse (Biotinylated, HEK293, His-Avi) is the recombinant mouse-derived GFRAL protein, expressed by HEK293 , with C-Avi, N-His labeled tag.

研究背景

GFRAL 蛋白是一种脑干限制性受体,在调节食物摄入、能量消耗和体重以应对代谢和毒素引起的压力方面发挥着至关重要的作用。在与其配体 GDF15 结合后,GFRALRET 相互作用并激活 MAPK 和 AKT 信号传导通路。GFRAL 通过其胞外结构域与 GDF15 和 RET 相互作用,充当 GDF15 的受体,并在 GDF15 结合后通过与 RET 的相互作用介导细胞信号传导。值得注意的是,与 RET 的相互作用需要先前的 GDF15 结合。

种属

Mouse

表达系统

HEK293

标签

N-His;N-Avi

蛋白编号

Q6SJE0-1 (Q20-E350)

基因 ID
蛋白结构
N-term
His-Avi
GFRAL (Q20-E350)
Accession # Q6SJE0-1
C-term
同用名
GFR alpha-like; GFRAL; GRAL; C6orf144
分子量

48-60 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
GFRAL Protein, Mouse (Biotinylated, HEK293, His-Avi)
目录号:
HY-P700728
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