GGH/Glutamyl hydrolase gamma 蛋白, Human (HEK293, His)

GGH (Glutamyl hydrolase gamma) protein hydrolyzes polyglutamate sidechains in pteroylpolyglutamates, progressively removing gamma-glutamyl residues to produce pteroyl-alpha-glutamate (folic acid) and free glutamate. Studies indicate GGH's crucial role in modulating the bioavailability of dietary pteroylpolyglutamates and its significance in the metabolism of both pteroylpolyglutamates and antifolates. GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is the recombinant human-derived GGH/Glutamyl hydrolase gamma protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of GGH/Glutamyl hydrolase gamma Protein, Human (HEK293, His) is 294 a.a., with molecular weight of ~40 KDa.

GGH（谷氨酰水解酶 γ）蛋白有助于水解蝶酰聚谷氨酸中存在的聚谷氨酸侧链。其酶活性涉及从蝶酰聚-γ-谷氨酸中逐步去除γ-谷氨酰残基，从而产生蝶酰-α-谷氨酸（叶酸）和游离谷氨酸。研究阐明的这一过程表明，GGH 可能在调节膳食蝶酰聚谷氨酸的生物利用度方面发挥着关键作用，并且在蝶酰聚谷氨酸和抗叶酸剂的代谢中也发挥着重要作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-His;C-6*His

Q92820/NP_003869.1(M25-D318)

8836  [NCBI]

RPHGDTAKKPIIGILMQKCRNKVMKNYGRYYIAASYVKYLESAGARVVPVRLDLTEKDYEILFKSINGILFPGGSVDLRRSDYAKVAKIFYNLSIQSFDDGDYFPVWGTCLGFEELSLLISGECLLTATDTVDVAMPLNFTGGQLHSRMFQNFPTELLLSLAVEPLTANFHKWSLSVKNFTMNEKLKKFFNVLTTNTDGKIEFISTMEGYKYPVYGVQWHPEKAPYEWKNLDGISHAPNAVKTAFYLAEFFVNEARKNNHHFKSESEEEKALIYQFSPIYTGNISSFQQCYIFD

Approximately 40 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.