GPNMB/Osteoactivin 蛋白, Human (Isoform 2, HEK293, Fc)

GPNMB, also known as Osteoactivin, is suggested to potentially function as a melanogenic enzyme. GPNMB/Osteoactivin Protein, Human (Isoform 2, HEK293, Fc) is the recombinant human-derived GPNMB/Osteoactivin protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GPNMB/Osteoactivin Protein, Human (Isoform 2, HEK293, Fc) is 453 a.a., with molecular weight of ~107.5 kDa.

GPNMB，也称为骨激活素，被认为具有潜在的黑色素生成酶功能。

Measured  by its binding ability in a functional ELISA. Immobilized Recombinant Human  Integrin alpha V beta 3 at 2 μg/mL (100 μL/well) can bind Biotinylated Human  GPNMB with an ED₅₀of 1.557 µg/mL.

Human

HEK293

C-hFc

Q14956-2 (A22-P474)

10457  [NCBI]

AKRFHDVLGNERPSAYMREHNQLNGWSSDENDWNEKLYPVWKRGDMRWKNSWKGGRVQAVLTSDSPALVGSNITFAVNLIFPRCQKEDANGNIVYEKNCRNEAGLSADPYVYNWTAWSEDSDGENGTGQSHHNVFPDGKPFPHHPGWRRWNFIYVFHTLGQYFQKLGRCSVRVSVNTANVTLGPQLMEVTVYRRHGRAYVPIAQVKDVYVVTDQIPVFVTMFQKNDRNSSDETFLKDLPIMFDVLIHDPSHFLNYSTINYKWSFGDNTGLFVSTNHTVNHTYVLNGTFSLNLTVKAAAPGPCPPPPPPPRPSKPTPSLGPAGDNPLELSRIPDENCQINRYGHFQATITIVEGILEVNIIQMTDVLMPVPWPESSLIDFVVTCQGSIPTEVCTIISDPTCEITQNTVCSPVDVDEMCLLTVRRTFNGSGTYCVNLTLGDDTSLALTSTLISVP

Approximately 107.5 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.