GSK-3 beta 蛋白, Human (sf9, His)

GSK-3β is a serine-threonine kinase and negative regulator of glucose homeostasis. GSK-3β has been implicated in neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. GSK-3β is widely expressed in multiple tissues, with particularly high levels in the brain and thyroid gland. GSK-3 beta Protein, Human (sf9, His) is the recombinant human-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-His labeled tag.

GSK-3 β 蛋白是糖原合成酶激酶亚家族中的一种丝氨酸-苏氨酸激酶，作为葡萄糖稳态的负调节因子，在能量代谢、炎症、内质网应激、线粒体功能障碍和细胞凋亡途径中发挥着至关重要的作用。该基因的缺陷与帕金森病和阿尔茨海默病有关，在神经退行性疾病中具有重要作用。GSK-3 β 在大脑 (RPKM 13.9)、甲状腺 (RPKM 9.9) 和 25 个其他组织中广泛表达。

1. The specific activity was determined to be > 45 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized His-GSK3B at 10 μg/ml (100 μl/well) can bind biotinylated human HG3C-CTNNB1, EC₅₀ of biotinylated human HG3C-CTNNB1is 0.15-0.35 μg/ml.

Human

Sf9 insect cells

N-His

P49841-2/NP_002084.2 (M1-T433)

2932  [NCBI]

MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKDSSGTGHFTSGVRVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASTPTNATAASDANTGDRGQTNNAASASASNST

44-48 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 25% glycerol, 0.5 mM PMSF, 0.5 mM EDTA.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.