GSK-3 beta 蛋白, Mouse (sf9, His)

GSK-3 beta is a protein kinase that regulates cellular circadian rhythm, autophagy, and apoptosis. GSK-3 beta Protein, Mouse (sf9, His) is the recombinant mouse-derived GSK-3 beta protein, expressed by Sf9 insect cells , with N-10*His labeled tag. The total length of GSK-3 beta Protein, Mouse (sf9, His) is 420 a.a., with molecular weight of ~47 kDa.

GSK-3 β 蛋白是一种组成型活性激酶，在葡萄糖稳态的激素控制、Wnt 信号传导以及转录因子和微管的调节中充当负调节因子。它通过磷酸化和灭活关键底物来实现这一点，例如糖原合酶（GYS1 或 GYS2）、EIF2B、CTNNB1/β-连环蛋白、APC、AXIN1、DPYSL2/CRMP2、JUN、NFATC1/NFATC、MAPT/TAU 和 MACF1。引物磷酸化是其大多数底物的先决条件。在骨骼肌中，GSK-3 beta 通过抑制 GYS1 活性来促进胰岛素对糖原合成的调节。它还可以通过调节转录因子的激活来介导胰岛素抵抗。此外，GSK-3 beta 通过控制起始因子 2B (EIF2BE/EIF2B5) 的活性在蛋白质合成中发挥作用。在 Wnt 信号传导中，它与 APC、AXIN1 和 CTNNB1/β-连环蛋白形成多聚复合物，磷酸化 CTNNB1 并通过泛素/蛋白酶体靶向其降解。GSK-3 beta 参与多种细胞过程，包括调节胰腺 β 细胞的复制、影响细胞凋亡、参与 ERBB2 依赖性微管稳定以及控制细胞极性和轴突生长。它还在生物钟调节、自噬和外源性凋亡信号通路的负调节中发挥作用。此外，GSK-3 β 磷酸化多种蛋白质，包括 E2F1、FXR1 和白细胞介素 22 受体亚基 IL22RA1，影响其稳定性和功能。

1.The specific activity was determined to be > 20 nmol/min/mg using synthetic Phospho-Glycogen Synthase Peptide-2 (YRRAAVPPSPSLSRHSSPHQpSEDEEE) as substrate.
2. Immobilized GSK-3 beta Protein, Mouse (sf9, His) at 10 μg/mL (100 μl/well) can bind human HG3C-CTNNB1 and the EC₅₀ is 0.15-0.35 μg/mL.

Mouse

Sf9 insect cells

N-10*His

Q9WV60 (M1-T420)

56637  [NCBI]

MSGRPRTTSFAESCKPVQQPSAFGSMKVSRDKDGSKVTTVVATPGQGPDRPQEVSYTDTKVIGNGSFGVVYQAKLCDSGELVAIKKVLQDKRFKNRELQIMRKLDHCNIVRLRYFFYSSGEKKDEVYLNLVLDYVPETVYRVARHYSRAKQTLPVIYVKLYMYQLFRSLAYIHSFGICHRDIKPQNLLLDPDTAVLKLCDFGSAKQLVRGEPNVSYICSRYYRAPELIFGATDYTSSIDVWSAGCVLAELLLGQPIFPGDSGVDQLVEIIKVLGTPTREQIREMNPNYTEFKFPQIKAHPWTKVFRPRTPPEAIALCSRLLEYTPTARLTPLEACAHSFFDELRDPNVKLPNGRDTPALFNFTTQELSSNPPLATILIPPHARIQAAASPPANATAASDTNAGDRGQTNNAASASASNST

Approximately 47 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 25% glycerol, 0.2 mM DTT, pH 7.4

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.