GSK3α 蛋白, Human (Sf9, GST)

GSK3B is a protein involved in various cellular processes. It regulates glycogen synthesis, Wnt signaling, apoptosis, autophagy, and neuronal functions. It phosphorylates and deactivates specific proteins, impacting their activity and function in the cell. GSK3α Protein, Human (Sf9, GST) is the recombinant human-derived GSK3α protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of GSK3α Protein, Human (Sf9, GST) is 482 a.a., with molecular weight of ~77.5 kDa.

GSK3α 蛋白是一种组成型活性激酶，作为负调节因子在葡萄糖稳态、Wnt 信号传导以及转录因子和微管调节的激素控制中发挥着至关重要的作用。它通过磷酸化和灭活关键靶点（如糖原合酶（GYS1 或 GYS2）、CTNNB1/β-连环蛋白、APC 和 AXIN1）来发挥其调节功能。引发的磷酸化是其大多数底物所必需的。GSK3α 通过抑制 GYS1 活性进而抑制糖原合成（尤其是肝脏中的糖原合成），从而有助于胰岛素控制糖原合成。此外，它还参与胰岛素抵抗的调节，并通过调节核 CTNNB1/β-连环蛋白的水平和转录活性在 Wnt 信号传导中发挥作用。GSK3α 还参与与阿尔茨海默病相关的淀粉样前体蛋白 (APP) 加工，调节胰腺 β 细胞的复制，对于神经元极性的建立和轴突生长至关重要。此外，它响应生长因子剥夺而控制细胞凋亡，负向调节外源性凋亡信号通路，并促进死亡受体处抗凋亡复合物的形成。GSK3α 还通过介导饥饿条件下 KAT5/TIP60 的磷酸化，促进关键自噬调节因子的乙酰化，充当自噬调节因子。此外，它还通过磷酸化 RICTOR 来负向调节 mTORC2 复合物，导致其泛素化和降解。

The activity of GSK3a protein is determined using the MSA method, which assesses its ability to phosphorylate fluorescent peptides within 30 minutes.

Human

Sf9 insect cells

N-GST

P49840 (S2-S483)

2931

Approximately 77.5 kDa

Greater than 80% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M Trehalose, pH7.5.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.