Kras4B 蛋白, Human (G12V, His)

The Kras4B protein interacts specifically with GPR31, dependent on farnesylation. This binding suggests a regulatory role for Kras4B in association with GPR31, emphasizing the importance of the farnesylation process. Comprehensive exploration into the molecular details of this interaction is crucial to understand the precise mechanisms and functional implications in cellular processes or signaling pathways. Kras4B Protein, Human (G12V, His) is the recombinant human-derived Kras4B protein, expressed by E. coli , with N-6*His labeled tag. The total length of Kras4B Protein, Human (G12V, His) is 150 a.a., with molecular weight of ~24.0 kDa.

Kras4B 蛋白与 GPR31 建立特异性相互作用，并且这种结合取决于法呢基化。这种相互作用强调了 Kras4B 与 GPR31 结合的潜在调节作用，表明对法尼基化过程的依赖性。进一步探索这种相互作用的分子复杂性对于阐明与 Kras4B 和 GPR31 在细胞过程或信号通路中相互作用相关的精确机制和功能意义至关重要。

Measured by its ability to catalyze the substrate GTP. The specific activity is 2.57 nmol/min/mg, as measured under the described conditions.

Human

E. coli

N-6*His

P01116-2 (M1-Q150)

3845  [NCBI]

MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK

Approximately 24.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.