HAI-1 蛋白, Human (His)

HAI-1 Protein serves as an inhibitor of HGFAC, suppressing serine protease activity of ST14/matriptase in vitro and inhibiting TMPRSS13 through the BPTI/Kunitz inhibitor 1 domain. It interacts with HGFAC and TMPRSS13, with the latter interaction promoting TMPRSS13 phosphorylation and cell membrane localization. HAI-1 Protein, Human (His) is the recombinant human-derived HAI-1 protein, expressed by HEK293 , with C-His labeled tag.

HAI-1 蛋白作为 HGFAC 抑制剂发挥作用，并对 ST14/matriptase 的丝氨酸蛋白酶活性具有体外抑制作用。通过其 BPTI/Kunitz 抑制剂 1 结构域，HAI-1 还阻碍 TMPRSS13 的丝氨酸蛋白酶活性。HAI-1 与 HGFAC 和 TMPRSS13 相互作用，形成影响细胞过程的动态关联。与 HGFAC 的相互作用表明该蛋白酶的激活具有调节作用，而与 TMPRSS13 的相互作用促进 TMPRSS13 的磷酸化和细胞膜定位。这些复杂的相互作用和抑制功能突出了 HAI-1 在调节丝氨酸蛋白酶活性中的多功能调节作用，强调了其在细胞稳态和蛋白水解途径中的重要性。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC₅₀ is < 1 nM.

Human

HEK293

C-His

O43278-2 (G36-V433)

6692  [NCBI]

GPPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGPSMERRHPVCSGTCQPTQFRCSNGCCIDSFLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDV

50-70 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.