2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Hepsin/HPN 蛋白, Mouse (HEK293, His)

Hepsin/HPN 蛋白, Mouse (HEK293, His)

目录号: HY-P77961
产品使用指南

Hepsin/HPN 是一种丝氨酸蛋白酶，可裂解细胞外底物，对 HGF 和 MST1/HGFL 等生长因子的加工有显着贡献。它在细胞生长和形态维持中起着至关重要的作用。Hepsin/HPN 蛋白, Mouse (HEK293, His) 是重组的 Hepsin/HPN 蛋白，由 HEK293 表达，带有 C-His 标签。Hepsin/HPN 蛋白, Mouse (HEK293, His) 全长 377 个氨基酸，分子量分别为 18-22 kDa & 30 kDa。

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50 μg   询价  
100 μg   询价  

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描述

Hepsin/HPN is a serine protease that cleaves extracellular substrates and contributes significantly to the processing of growth factors such as HGF and MST1/HGFL. It plays a vital role in cell growth and morphology maintenance. Hepsin/HPN Protein, Mouse (HEK293, His) is the recombinant mouse-derived Hepsin/HPN protein, expressed by HEK293 , with C-His labeled tag. The total length of Hepsin/HPN Protein, Mouse (HEK293, His) is 377 a.a., with molecular weight of 18-22 kDa & 30 kDa, respectively.
研究背景

Hepsin/HPN 是一种丝氨酸蛋白酶，是细胞外底物裂解中的关键酶，对 HGF 和 MST1/HGFL 等生长因子的蛋白水解过程有显着贡献。它的参与延伸到关键的细胞功能，在细胞生长和细胞形态的维持中发挥着至关重要的作用。此外，Hepsin/HPN 参与 ACE2 的蛋白水解过程，表明其参与重要的细胞途径。值得注意的是，这种蛋白酶还介导尿 UMOD 的裂解，这是 UMOD 聚合的一个不可或缺的步骤，突出了其在调节各种生物过程中的多种功能。
种属

Mouse
表达系统

HEK293
标签

C-His
蛋白编号

O35453 (T60-P436)
基因 ID
蛋白结构
N-term
Hepsin (T60-P436)
Accession # O35453
His
C-term
同用名
Hpxn; FLJ56652; Hemopexin; HPX; HX; Hepsin; HPN; TMPRSS1
分子量

18-22 kDa&30 kDa
纯度

Greater than 95% as determined by Tris-Bis PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Hepsin/HPN Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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