HER2/CD340 蛋白, Rat (HEK293, His)

The HER2/CD340 protein is a multifunctional tyrosine kinase that is a component of the neuregulin receptor complex and regulates microtubule dynamics.Activated HER2/CD340 triggers the MEMO1-RHOA-DIAPH1 pathway, inhibits GSK3B on the cell membrane and promotes the binding of APC and CLASP2, which is critical for microtubule stability.HER2/CD340 Protein, Rat (HEK293, His) is the recombinant rat-derived HER2/CD340 protein, expressed by HEK293 , with C-10*His labeled tag.

HER2/CD340 蛋白是一种多面蛋白酪氨酸激酶，是各种细胞表面受体复合物的重要组成部分，需要辅助受体来进行配体结合。它是神经调节蛋白-受体复合物的组成部分，与神经调节蛋白协同作用，并且 GP30 成为该受体的潜在配体。除了其受体作用之外，HER2/CD340 在调节外周微管 (MT) 的生长和稳定方面也发挥着关键作用。激活后，由 ERBB2 激活触发的 MEMO1-RHOA-DIAPH1 信号通路会导致 GSK3B 磷酸化并随后在细胞膜上受到抑制。这种精心策划的抑制可防止 APC 和 CLASP2 的磷酸化，促进它们与细胞膜的结合。膜结合的 APC 反过来又促进 MACF1 定位到细胞膜，这是微管捕获和稳定的关键步骤。在细胞核内，HER2/CD340 参与转录调控，与 PTGS2/COX-2 启动子中的 5'-TCAATTC-3' 序列结合并激活其转录。此外，它还通过 RNA Pol I 参与 rRNA 基因的转录，增强蛋白质合成并促进细胞生长。HER2/CD340 的多方面功能强调了其在多种细胞过程中的核心作用，从受体信号传导到微管动力学和转录调节。

Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED₅₀ this effect is ≤1.043 μg/mL in the presence of 0.6 µg/mL Trastuzmab, corresponding to a specific activity is ≥9.59×10² units/mg.

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  Measured by its ability to block anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer cell proliferation. The ED₅₀ for this effect is 1.043 μg/mL in the presence of 0.6 µg/mL Trastuzmab, corresponding to a specific activity is 9.59×10² units/mg.

Rat

HEK293

C-10*His

AAH61863 (M4-T656)

24337  [NCBI]

MELAAWCRWGFLLALLPPGIAGTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYVPANASLSFLQDIQEVQGYMLIAHNQVKRVPLQRLRIVRGTQLFEDKYALAVLDNRDPQDNVAASTPGRTPEGLRELQLRGLTEILKGGVLIRGNPQLCYQDMVLWKDVFRKNNQLAPVDIDTNRSRACPPCAPACKDNHCWGESPEDCQILTGTICTSGCARCKGRLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMHNPEGRYTFGASCVTTCPYNYLSTEVGSCTLVCPPNNQEVTAEDGTQRCEKCSKPCARVCYGLGMEHLRGARAITSDNVQEFDGCKKIFGSLAFLPESFDGDPSSGIAPLRPEQLQVFETLEEITGYLYISAWPDSLRDLSVFQNLRIIRGRILHDGAYSLTLQGLGIHSLGLRSLRELGSGLALIHRNAHLCFVHTVPWDQLFRNPHQALLHSGNRPEEDCGLEGLVCNSLCAHGHCWGPGPTQCVNCSHFLRGQECVEECRVWKGLPREYVSDKRCLPCHPECQPQNSSETCFGSEADQCAACAHYKDSSSCVARCPSGVKPDLSYMPIWKYPDEEGICQPCPINCTHSCVDLDERGCPAEQRASPVT

Approximately 91 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.