HEXB/Hexosaminidase B 蛋白, Mouse (HEK293, His)

HEXB, or Hexosaminidase B protein, hydrolyzes N-acetyl-D-hexosamine in glycoconjugates. It selectively acts on neutral oligosaccharides, not degrading other substrates. HEXB does not degrade GM2 gangliosides; only isozyme A does. During fertilization, HEXB contributes to the zona block to polyspermy by inactivating the sperm galactosyltransferase-binding site. This mechanism prevents sperm binding to the zona pellucida. HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is the recombinant mouse-derived HEXB/Hexosaminidase B protein, expressed by HEK293 , with C-His labeled tag. The total length of HEXB/Hexosaminidase B Protein, Mouse (HEK293, His) is 505 a.a., with molecular weight of ~59.4 kDa.

HEXB 或氨基己糖苷酶 B 蛋白，在水解糖复合物中的非还原端 N-乙酰基-D-己糖胺和/或硫酸化 N-乙酰基-D-己糖胺方面发挥着至关重要的作用。这包括来自蛋白质、中性糖脂和某些粘多糖的寡糖部分。有趣的是，同工酶 B 表现出选择性底物特异性，可有效水解中性寡糖，同时不作用于其他底物。值得注意的是，在 GM2A 存在的情况下，只有同工酶 A 负责 GM2 神经节苷脂的降解。此外，在受精过程中，HEXB 存在于未激活卵母细胞的皮质颗粒中，有助于多精卵的透明带阻断。卵母细胞激活后，HEXB 在皮质反应期间被胞吐，有效地灭活精子半乳糖基转移酶结合位点。研究报告称，这种分子机制解释了精子与透明带结合受阻的原因。

Measured by its ability to hydrolyze 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide (4-MU-GlcNAc). Read at excitation and emission wavelengths of 365 nm and 445 nm (top read). The specific activity is 3033.81 pmol/min/µg, as measured under the described conditions.

Mouse

HEK293

C-His

P20060 (Q32-I536)

15212  [NCBI]

QPALWPFPRSVQMFPRLLYISAEDFSIDHSPNSTAGPSCSLLQEAFRRYYNYVFGFYKRHHGPARFRAEPQLQKLLVSITLESECESFPSLSSDETYSLLVQEPVAVLKANSVWGALRGLETFSQLVYQDSFGTFTINESSIADSPRFPHRGILIDTSRHFLPVKTILKTLDAMAFNKFNVLHWHIVDDQSFPYQSTTFPELSNKGSYSLSHVYTPNDVRMVLEYARLRGIRVIPEFDTPGHTQSWGKGQKNLLTPCYNQKTKTQVFGPVDPTVNTTYAFFNTFFKEISSVFPDQFIHLGGDEVEFQCWASNPNIQGFMKRKGFGSDFRRLESFYIKKILEIISSLKKNSIVWQEVFDDKVELQPGTVVEVWKSEHYSYELKQVTGSGFPAILSAPWYLDLISYGQDWKNYYKVEPLNFEGSEKQKQLVIGGEACLWGEFVDATNLTPRLWPRASAVGERLWSPKTVTDLENAYKRLAVHRCRMVSRGIAAQPLYTGYCNYENKI

Approximately 59.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.