HGF 蛋白, Mouse (HEK293, His)

HGF Protein, a potent mitogen, stimulates mature hepatocyte cell growth and acts as a hepatotrophic factor for various tissues. As an activating ligand for MET receptor tyrosine kinase, HGF binds and promotes dimerization, activating MAPK signaling through TMPRSS13 cleavage. Structurally, HGF comprises alpha and beta chains connected by a disulfide bond. Interaction with SRPX2 enhances its mitogenic activity. HGF Protein, Mouse (HEK293, His) is the recombinant mouse-derived HGF protein, expressed by HEK293 , with C-6*His labeled tag.

HGF 蛋白是一种有效的有丝分裂原，可刺激成熟肝实质细胞的生长，并作为多种组织和细胞类型的肝营养因子。它充当受体酪氨酸激酶 MET 的激活配体，与其结合并促进其二聚化。HGF 还通过切割和激活 TMPRSS13 来激活 MAPK 信号传导。在结构上，HGF由通过二硫键连接的α链和β链组成。此外，它与 SRPX2 相互作用，促进 HGF 的促有丝分裂活性增加。

Measured in a cell proliferation assay using mIMCD-3 mouse epithelial cells. The ED₅₀ this effect is 11.8-12.06 ng/mL.

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  Measured in a cell proliferation assay using mIMCD‑3 mouse epithelial cells. The ED₅₀ for this effect is 11.8 ng/mL, corresponding to a specific activity is 8.47×10⁴ units/mg.

Mouse

HEK293

C-6*His

Q08048-1 (Q33-L728)

15234  [NCBI]

QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKKVNSADECANRCIRNRGFTFTCKAFVFDKSRKRCYWYPFNSMSSGVKKGFGHEFDLYENKDYIRNCIIGKGGSYKGTVSITKSGIKCQPWNSMIPHEHSFLPSSYRGKDLQENYCRNPRGEEGGPWCFTSNPEVRYEVCDIPQCSEVECMTCNGESYRGPMDHTESGKTCQRWDQQTPHRHKFLPERYPDKGFDDNYCRNPDGKPRPWCYTLDPDTPWEYCAIKTCAHSAVNETDVPMETTECIQGQGEGYRGTSNTIWNGIPCQRWDSQYPHKHDITPENFKCKDLRENYCRNPDGAESPWCFTTDPNIRVGYCSQIPKCDVSSGQDCYRGNGKNYMGNLSKTRSGLTCSMWDKNMEDLHRHIFWEPDASKLNKNYCRNPDDDAHGPWCYTGNPLIPWDYCPISRCEGDTTPTIVNLDHPVISCAKTKQLRVVNGIPTQTTVGWMVSLKYRNKHICGGSLIKESWVLTARQCFPARNKDLKDYEAWLGIHDVHERGEEKRKQILNISQLVYGPEGSDLVLLKLARPAILDNFVSTIDLPSYGCTIPEKTTCSIYGWGYTGLINADGLLRVAHLYIMGNEKCSQHHQGKVTLNESELCAGAEKIGSGPCEGDYGGPLICEQHKMRMVLGVIVPGRGCAIPNRPGIFVRVAYYAKWIHKVILTYKL

(94-99)&(63-65)&(30-34) kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.