HGFA/HGF Activator 蛋白, Human (HEK293, His)

HGFA/HGF-activating proteins play a crucial role by catalyzing the activation of hepatocyte growth factor (HGF) and converting it into biologically active heterodimers. The process involves the formation of heterodimers with short and long chains linked by disulfide bonds. HGFA/HGF Activator Protein, Human (HEK293, His) is the recombinant human-derived HGFA/HGF Activator protein, expressed by HEK293 , with C-His labeled tag.

HGFA/HGF 激活剂是细胞过程中的关键酶，因为它通过将肝细胞生长因子 (HGF) 从单链形式转化为具有生物活性的异二聚体构型来催化其激活。这种激活机制涉及异二聚体的形成，该异二聚体由短链和长链组成，通过二硫键错综复杂地连接。这种异二聚体结构强调了 HGFA 在调节 HGF 活性中的重要作用，暗示其在各种细胞反应和信号传导途径中的重要性。

Measured by its ability to cleave 10 μM peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 that incubate at room temperature for 30 minutes .The specific activity is 33.56 pmol/min/µg.

Human

HEK293

C-His

Q04756/NP_001519.1 (Q36-S655)

3083  [NCBI]

Approximately 34&37&65&105 kDa.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.