HHLA2 蛋白, Human (HEK293, Fc)

HHLA2 protein, a key immune regulator, interacts with TMIGD2, providing crucial costimulation for T-cells during TCR-mediated activation. This interaction amplifies T-cell proliferation and cytokine production through an AKT-dependent signaling cascade, revealing HHLA2's role in enhancing T-cell responses and immune modulation. HHLA2 Protein, Human (HEK293, Fc) is the recombinant human-derived HHLA2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of HHLA2 Protein, Human (HEK293, Fc) is 344 a.a., with molecular weight of ~63.7 KDa.

HHLA2 蛋白是免疫调节中的关键参与者，它与 TMIGD2 发生显着的相互作用，在 T 细胞受体 (TCR) 介导的激活过程中为 T 细胞提供共刺激。这种相互作用反过来又通过 AKT 依赖性信号级联放大 T 细胞增殖和细胞因子产生。HHLA2 和 TMIGD2 之间的合作强调了增强 T 细胞反应所涉及的复杂分子机制，揭示了其在免疫调节中的作用。

1.Measured by its binding ability in a functional ELISA.
2.Immobilized human TMIGD2 at 10 μg/mL (100 μL/well) can bind human HHLA2-Fc, the EC₅₀ of human HHLA2-Fc is 0.3-3 μg/mL.
3.Loaded Recombinant Human KIR3DL3 Protein, His Tag on His1K Biosensor, can bind Recombinant Human B7-H7/HHLA2 Protein, hFc Tag with an affinity constant of 51.8 nM as determined in BLI assay (Sartorius Octet RED384)

Human

HEK293

C-hFc

Q9UM44 (M1-N344)

11148  [NCBI]

Approximately 63.7 kDa.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.