Histone H3 蛋白, Human (His)

Histone H3 proteins are critical in nucleosomes, which compact DNA into chromatin and regulate DNA accessibility. Histone H3 Protein, Human (His) is the recombinant human-derived Histone H3 protein, expressed by E. coli , with N-His labeled tag. The total length of Histone H3 Protein, Human (His) is 136 a.a., with molecular weight of ~16.8 kDa.

组蛋白 H3 是核小体的基本组成部分，在将 DNA 包裹和压缩到染色质的复杂过程中发挥着关键作用，这反过来又限制了 DNA 进入需要 DNA 作为模板的细胞机器。这种组蛋白及其对应物在关键细胞功能中发挥着关键作用，包括转录调节、DNA 修复、DNA 复制和染色体稳定性的维持。DNA 可及性的调节涉及翻译后修饰（统称为组蛋白密码）的复杂相互作用，以及核小体的动态重塑。核小体本身包含组蛋白八聚体，由 H2A、H2B、H3 和 H4 各两个分子组成，组装成一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体。这种八聚体有效地包裹了大约 147 个碱基对的 DNA，证明了它在组织染色质结构和促进关键基因组过程中不可或缺的作用。此外，组蛋白 H3 与各种细胞成分（例如 TONSL、CHAF1A、CHAF1B、MCM2 和 DNAJC9）相互作用，进一步促进其多方面功能。

Human

E. coli

N-His

P68431 (A2-A136)

8350  [NCBI]

ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEACEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Approximately 18 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, 200 mM arginine, pH 8.0.

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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.