Histone H3 蛋白, Xenopus laevis (98a.a, His)

Histone H3 Protein constitutes a core element in the nucleosome, an octamer comprising H2A, H2B, H3, and H4. The assembly forms a histone octamer with one H3-H4 heterotetramer and two H2A-H2B heterodimers, serving as a molecular spool that wraps around 147 base pairs of DNA. This compact organization contributes to chromatin structure, and Histone H3, as part of this assembly, plays a crucial role in chromatin structure and gene regulation. Histone H3 Protein, Xenopus laevis (98a.a, His) is the recombinant Xenopus laevis-derived Histone H3 protein, expressed by E. coli , with tag free. The total length of Histone H3 Protein, Xenopus laevis (98a.a, His) is 98 a.a..

组蛋白 H3 蛋白是核小体的基本成分，由组蛋白八聚体组成，其中每个分子包含 H2A、H2B、H3 和 H4 两个分子。该组装体由一个 H3-H4 异四聚体和两个 H2A-H2B 异二聚体组成，共同形成八聚体核心。组蛋白八聚体起到分子线轴的作用，将大约 147 个 DNA 碱基对包裹在自身周围，有助于染色质的紧凑组织。组蛋白 H3 作为该组装体的一部分，属于组蛋白 H3 家族，在染色质结构和基因调控中发挥着关键作用。

Xenopus laevis

E. coli

N-6*His

Q92133 (P39-A136)

399088  [NCBI]

PHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVALFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA

Approximately 13 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 4mM HCL, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.