1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. HK3/Hexokinase-3 蛋白, Human (sf9, His-GST)

HK3/Hexokinase-3 蛋白, Human (sf9, His-GST)

目录号: HY-P74881
产品使用指南

HK3(己糖激酶-3)蛋白促进己糖(即D-葡萄糖和D-果糖)磷酸化形成6-磷酸己糖(具体为D-葡萄糖6-磷酸和6-磷酸),起着至关重要的作用在细胞代谢中。D-果糖6-磷酸。HK3/Hexokinase-3 蛋白, Human (sf9, His-GST) 是重组的 HK3/己糖激酶 3 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。HK3/Hexokinase-3 蛋白, Human (sf9, His-GST) 全长 923 个氨基酸,分子量约为 ~115 kDa。

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描述

The HK3 (hexokinase-3) protein promotes the phosphorylation of hexoses (i.e., D-glucose and D-fructose) to form hexose-6-phosphate (specifically, D-glucose 6-phosphate and 6-phosphate), Plays a vital role in cell metabolism. D-fructose 6-phosphate. HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is the recombinant human-derived HK3/Hexokinase-3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is 923 a.a., with molecular weight of ~115 kDa.

研究背景

HK3蛋白,也称为己糖激酶-3,通过催化己糖(包括D-葡萄糖和D-果糖)磷酸化形成6-磷酸己糖,例如D-葡萄糖6-磷酸和D-葡萄糖,在糖酵解中发挥着至关重要的作用。分别为果糖6-磷酸。该酶介导糖酵解的初始步骤和限速步骤,促进 D-葡萄糖转化为 D-葡萄糖 6-磷酸。通过这种酶活性,HK3 有助于葡萄糖进入糖酵解途径,这是细胞能量代谢的基本过程。HK3 对己糖的磷酸化对于细胞内随后的代谢途径和能量产生至关重要。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

P52790 (M1-V923)

基因 ID
蛋白结构
N-term
His-GST
HK3 (M1-V923)
Accession # P52790
C-term
同用名
Hexokinase-3; HK III; Hexokinase-C; HK3
分子量

Approximately 115 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

HK3/Hexokinase-3 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
HK3/Hexokinase-3 Protein, Human (sf9, His-GST)
目录号:
HY-P74881
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