Human IL-23 alpha & Mouse IL-12 beta Heterodimer 蛋白 (HEK293, His)

IL-23 and IL12B together constitute the pro-inflammatory cytokine IL-23, which is crucial in innate and adaptive immunity. IL-23 is released by antigen-presenting cells such as dendritic cells or macrophages, binds to IL12RB1 and IL23R, and activates JAK2 and TYK2. Human IL-23 alpha & Mouse IL-12 beta Heterodimer Protein (HEK293, His) is a recombinant protein dimer complex containing mouse, human-derived Human IL-23 alpha & Mouse IL-12 beta Heterodimer protein, expressed by HEK293 , with C-6*His labeled tag.

IL-23 与 IL12B 协同形成促炎细胞因子 IL-23，在先天免疫和适应性免疫中发挥多种作用。IL-23 由树突状细胞或巨噬细胞等抗原呈递细胞释放，与包含 IL12RB1 和 IL23R 的异二聚体受体复合物结合，启动涉及 JAK2 和 TYK2 激活的级联反应。这些激酶磷酸化受体，为随后的 STAT3 和 STAT4 磷酸化创建对接位点。该过程激活多种途径，包括 p38 MAPK 或 NF-kappa-B，促进促炎细胞因子的产生，例如 IL-17A/IL17A。此外，IL-23 积极参与细胞内细菌的早期有效清除。值得注意的是，IL-23 促进 T 辅助细胞 17 细胞的扩增和存活，T 辅助细胞 17 是一种以产生 IL-17 的 CD4 阳性辅助 T 细胞亚群，以及其他产生 IL-17 的细胞。IL-23 与 IL12B 的异二聚体（称为白细胞介素 IL-23）是二硫键连接的。此外，IL-23 与 IL23R 相互作用，促进 IL12RB1 的募集。

Measured by its ability to induce IL-17 secretion by CTLL-2 cells. The ED50 for this effect is 2.703 ng/mL, corresponding to a specific activity is 3.7×10^5 U/mg.

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  Measured by its ability to induce IL-17 secretion by CTLL-2 cells.The ED50 for this effect is 2.703 ng/mL, corresponding to a specific activity is 3.7×10⁵ U/mg.

Mouse; Human

HEK293

C-6*His

Q9NPF7 (R20-P189)&P43432 (M23-S335)

51561  [NCBI]&16160  [NCBI]

RAVPGGSSPAWTQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDPQGLRDNSQFCLQRIHQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIPSLSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP&MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

Approximately 23.18 & 40-50 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.