1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-alpha
  5. IFN-alpha 5
  6. IFN-alpha 5/IFNA5 蛋白, Mouse (P.pastoris, His)

IFN-alpha 5/IFNA5 蛋白, Mouse (P.pastoris, His)

目录号: HY-P76460
COA 产品使用指南

IFN-alpha 5 (IFNA5) 属于 I 型干扰素家族,由巨噬细胞产生而具有抗病毒活性。重组小鼠 IFN-alpha 5/IFNA5 蛋白 (P.pastoris, His) 含有 168 个氨基酸 (C24-E189),由 P. pastoris 酵母细胞产生并带有 C 端 His 标签。

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规格 价格 是否有货 数量
20 μg ¥5500 In-stock
50 μg   询价  
100 μg   询价  

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描述

IFN-alpha 5 (IFNA5), belongs to type I interferon family, is produced by macrophages with antiviral activities[1]. IFN-alpha 5/IFNA5 Protein, Mouse (P.pastoris, His) contains 168 a.a. (C24-E189), produced in P. pastoris yeast cells with a C-terminal His-tag.

研究背景

干扰素-α5(IFNA5;IFN-α5)属于α/β干扰素(IFN)家族,由巨噬细胞产生,具有抗病毒活性。干扰素(IFN)最初被认为是一种在体外“干扰”病毒复制的物质。IFN-α/β及相关分子被归类为I型干扰素,其他两类分别为II型干扰素(IFN-γ)和III型干扰素(IFN-λ)[1]
干扰素刺激两种酶的产生:蛋白激酶和寡腺苷酸合成酶。干扰素 α (IFNa) 在多种癌症中显示出显着的生物活性,特别是血液系统恶性肿瘤,如毛细胞白血病和慢性粒细胞白血病[2]
IFN-α 5 参与先天免疫,并且是与呼吸道合胞病毒 (RSV) 引起的急性病毒性细支气管炎 (AVB) 相关的基因之一,决定了对 RSV 细支气管炎的易感性[3][4]
流感病毒感染过程中,过度表达的干扰素-α(IFN-α)可能会导致炎症反应失控,造成病理损伤。而IFN-α 5主要表达于低致病性传染性支气管炎病毒(IBV)感染者的呼吸道上皮细胞[5]
至于IFN在动物模型中的广泛使用,小鼠IFNA5蛋白的氨基酸序列与人类差异很大(60.32%)。

体外研究
(In Vitro)

IFN-α subtype shows dominance in mouse lungs infected with influenza viruses. IFN-α1, IFN-α5, and IFN-α9 are dominantly expressed in the lungs of the mice infected with H1N1 influenza viruses accompanied with the high-level production of CXCL1, more infiltrated neutrophils and more severe damage in mouse lungs[5].

体内研究
(In Vivo)

Type I interferons (IFNs) are produced early in response to viral infection and modulate adaptive immunity. Thus IFNA5 enhances the replication of murine cytomegalovirus (MCMV) and helps little about systemic murine cytomegalovirus (MCMV) infection and myocarditis[6].

种属

Mouse

表达系统

P. pastoris

标签

C-His

蛋白编号

Q810G2 (C24-E189)

基因 ID
蛋白结构
N-term
IFNA5 (C24-E189)
Accession # Q810G2
His
C-term
中文名
重组小鼠 IFN-alpha 5/IFNA5 蛋白 (P.pastoris, His)
同用名
Interferon alpha-5; Interferon alpha-61; Interferon alpha-G; LeIF G
分子量

Approximately 20.3 kDa.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IFN-alpha 5/IFNA5 Protein, Mouse (P.pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IFN-alpha 5/IFNA5 Protein, Mouse (P.pastoris, His)
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HY-P76460
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