IFN-gamma R1/CD119 蛋白, Rat (HEK293, Fc)

IFN-gamma R1 (CD119), one of the subunit of IFN-gamma receptor, is a receptor for IFN-gamma. IFN-gamma R1 forms the functional receptor with IFN-gamma R2. Upon binding with IFN-gamma, IFN-gamma R1 and IFN-gamma R2 oligomerize and transphosphorylate^[1]. Then, JAK1 and JAK2 are phosphorylated and activated, and STAT1 is recruited to the receptor complex. Phosphorylated STAT1 translocates to the nucleus, where it regulates the expression of IFN-responsive genes (e.g. CD54)^[2]. IFN-gamma R1/CD119 Protein, Rat (HEK293, Fc) is a recombinant mouse IFN-gamma R1 (M1-S241) with C-terminal hFc tag, which is produced in HEK293 cells.

IFN-γ R1 (CD119) 是 IFN-γ 受体的亚基之一，是 IFN-γ 的受体。IFN-γ R1 组成型表达于几乎所有细胞的表面^[1]。
IFN-γ R1 可以与 IFN-γ R2 结合形成功能性受体。与 IFN-γ 结合后，IFNγR1 和 IFNγR2 寡聚并转磷酸化^[1]。然后，JAK1 和 JAK2 被磷酸化并激活，STAT1 被招募到受体复合物中。IFNγR1 的磷酸化为 STAT1 创建了一个对接位点，并导致 STAT1 磷酸化。磷酸化的 STAT1 易位到细胞核，在那里调节 IFN 反应基因（例如 CD54）的表达。IFN-γ R1 缺陷与 IFN-γ 介导的免疫反应以及感染易感性增加有关。IFN-γ R1 信号通路对于激活癌细胞死亡和抑制癌症进展具有重要作用^[3]
不同物种中 IFNAR1 的氨基酸序列有所不同。大鼠 IFN-γ R1 与小鼠具有 73.5% 的氨基酸序列同一性。大鼠 IFN-gamma R1 与人 IFN-gamma R1 的氨基酸序列一致性为 49.89%。IFN-gamma R1 在抗菌、抗病毒和抗肿瘤反应中发挥着关键作用^[2]。

IFN-gamma R1 (murine, -2 μg/mL, 4 h) suppresses AP(+)-exosome-mediated NO synthesis in RAW264.7 cells^[4].

Measured by its binding ability in a functional ELISA. Immobilized Human IFN-gamma at 5 μg/mL (100 μL/well) can bind Rat IFN-gamma R1. The ED₅₀ for this effect is 0.1293 µg/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human IFN-gamma at 5 μg/mL (100 μL/well) can bind Rat IFN-gamma R1. The ED₅₀ for this effect is 0.1293 µg/mL

Rat

HEK293

C-hFc

Q6P6T3 (A17-S241)

116465  [NCBI]

ALMSTEDPKPPSVPAPTNVLITSYDLNPVVHWKHQNVSQAAVFTVQVKMYPDNWTDACTNIAHHYCNIYKHISYPDSSAWARVKAKVGQRESAYAQSEEFIMCRKGKVGPPGLDIGRKEDQLIVHIFHPKVNVSQETMFGDGNTCYTFDYTVFVKHYRSGEILHTEHSVLKEDCSETLCELNISVSTLNSNYCVSVVGKSSFWQVNTETSKDACIPFLHDDREES

65-90 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Castro F, et al. Interferon-Gamma at the Crossroads of Tumor Immune Surveillance or Evasion. Front Immunol. 2018 May 4;9:847.  [Content Brief]

  [2]. van de Vosse E, et al. IFN-γR1 defects: Mutation update and description of the IFNGR1 variation database. Hum Mutat. 2017 Oct;38(10):1286-1296.  [Content Brief]

  [3]. Ding H, et al. Role of interferon-gamma (IFN-γ) and IFN-γ receptor 1/2 (IFNγR1/2) in regulation of immunity, infection, and cancer development: IFN-γ-dependent or independent pathway. Biomed Pharmacother. 2022 Nov;155:113683.  [Content Brief]

  [4]. Goto Y, et al. Contribution of the exosome-associated form of secreted endoplasmic reticulum aminopeptidase 1 to exosome-mediated macrophage activation. Biochim Biophys Acta Mol Cell Res. 2018 Jun;1865(6):874-888.  [Content Brief]