IgJ 蛋白, Human (His)

The IgJ protein links IgM or IgA monomers, nucleating IgM pentamers and inducing IgA dimer or polymer formation. It also binds these immunoglobulins to the secretory component. As part of the sIgA complex, IgJ integrates with IgA monomers, the J chain, and the secretory component (PIGR proteolytic product). IgJ Protein, Human (His) is the recombinant human-derived IgJ protein, expressed by E. coli , with C-His labeled tag. The total length of IgJ Protein, Human (His) is 137 a.a., with molecular weight of ~26 kDa.

IgJ 蛋白在连接 IgM 或 IgA 的两个单体单元方面发挥着关键作用。在 IgM 的情况下，J 链连接的二聚体充当 IgM 五聚体的成核单元，而在 IgA 的情况下，它诱导二聚体和/或更大的聚合物的形成。此外，IgJ 促进这些免疫球蛋白与分泌成分的结合。它是分泌型 IgA (sIgA) 复合物的组成部分，该复合物包含两个、四个或五个 IgA 单体，以及两个额外的非 Ig 多肽，即 J 链和分泌成分（PIGR 的蛋白水解产物）。

Human

E. coli

C-His

P01591/NP_653247.1 (Q23-D159)

3512  [NCBI]

QEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD

Approximately 26 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.