IL-10 蛋白, Canine

IL-10 is the most important cytokine that inhibits pro-inflammatory responses and limits excessive immune responses in various autoimmune diseases. It belongs to the type 2 cytokine superfamily. IL-10 plays an important role in transmitting information, activating and regulating immune cells, mediating T and B cell activation, proliferation and differentiation, and in inflammatory responses. IL-10 can promote the proliferation and activation of CD8T⁺ cells, enhancing anti-tumor capabilities. IL-10 Protein, Canine is the recombinant canine-derived IL-10 protein, expressed by E. coli , with tag free. The total length of IL-10 Protein, Canine is 160 a.a..

IL-10 是抑制各种自身免疫性疾病中的促炎反应和限制过度免疫反应的最重要的细胞因子。IL-10 在传递信息，激活与调节免疫细胞，介导 T、B 细胞活化、增殖与分化及在炎症反应中起重要作用。IL-10 可抑制 TH1 细胞和抗原呈递细胞 (巨噬细胞和树突状细胞) 产生细胞因子。此外，IL-10 不仅直接抑制促炎性 TH17 细胞，而且对 CD4⁺Foxp3⁺ 调节性 T 细胞 (Tregs) 有直接影响，并促进其抑制功能。当 IL-10 与其受体复合物结合时，JAK1 (位于 IL-10Rα 链上) 和 Tyk2 (位于 IL-10Rβ 链上) 被激活。这进一步导致 STAT3 磷酸化和下游基因表达，如 SOCS-1 和 SOCS-3。IL-10 能通过下调单核细胞表面主要组织相容性抗原 Ⅱ 的表达，降低其抗原呈递作用，下调 T 淋巴细胞活性, 抑制炎性细胞的激活、迁移和粘附。同时，IL-10 也能抑制炎症因子的合成与释放。IL-10 能够促进 CD8T⁺ 细胞的增殖和活化，增强了抗肿瘤能力^[1]。

Measured in a cell proliferation assay using HepG2. The ED₅₀ for this effect is 3.15 ng/mL. corresponding to a specific activity is 3.17×10⁵ units/mg.

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  Measured in a cell proliferation assay using HepG2. The ED₅₀ for this effect is 3.15 ng/mL. corresponding to a specific activity is 3.17×10⁵ units/mg.

Canine

E. coli

Tag Free

XP_855560 (S20-I179)

403628  [NCBI]

SRHQSTLPEDDCTHFPASLPHMLRELRAAFGRVKTFFQMKDKLDNILLTGSLLEDFKSYLGCQALSEMIQFYLEEVMPRAENHDPDIKNHVNSLGEKLKTLRLRLRRCHRFLPCENKSKAVEQVKSAFSKLQEKGVYKAMSEFDIFINYIETYMTMRMKI

Approximately 18.9 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 10 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.