IL-10 蛋白, Mouse (CHO)

IL-10 Protein, Mouse (CHO) is a potent CHO cell derived anti-inflammatory cytokine results in increased immune-mediated demyelination in mice infected with a neurotropic coronavirus.

抗炎细胞因子白细胞介素 10 (IL-10) 是一种多效性细胞因子，在大多数寄生虫、细菌、病毒和真菌疾病期间大量产生。IL-10 通过抑制先天或非常早期的 T 细胞免疫反应发挥作用。IL-10 治疗可能是某些类型病毒性脑炎的有用辅助治疗。IL-10 主要通过抑制 II 类主要组织相容性复合体 (MHC) 和共刺激分子（例如 CD80/CD86）的表达以及促炎细胞因子和趋化因子（包括 IL-12）的产生来抑制巨噬细胞和树突状细胞 (DC) 功能。IL-10 还对 T 细胞有直接影响，抑制激活和细胞因子表达。高度激活的病毒特异性 T 细胞产生 IL-10，这增加了 IL-10 通过自分泌和旁分泌信号传导发挥作用的可能性，以限制疾病急性期的炎症^[1]。

The ED₅₀ is <0.2 ng/mL as measured by MC/9 cells.

Mouse

CHO

Tag Free

P18893 (S19-S178)

16153  [NCBI]

SRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS

21-23 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Trandem K, et al. Virally expressed interleukin-10 ameliorates acute encephalomyelitis and chronic demyelinationin coronavirus-infected mice. J Virol. 2011 Jul;85(14):6822-31.  [Content Brief]