IL-10 蛋白, Rat (CHO)

IL-10 Protein, Rat (CHO) is a CHO cell derived immunosuppressive cytokine produced by a variety of mammalian cell types including macophages, monocytes, B-cells, T-cells and keratinocytes.

Interleukin-10 是一种抗炎 Th1 免疫抑制细胞因子，其活性形式是非共价同二聚体，在结构和生物活性方面均表现出物种特异性。IL-10 的大鼠同源物与人 IL-10 在氨基酸序列水平上具有 73% 的同一性，并且除了人 IL-10 中存在的两个二硫键外，还具有第五个未配对的半胱氨酸 (cys-149) ）。通过细菌表达制备大鼠 IL-10，然后在谷胱甘肽氧化还原系统中溶解和重折叠，产生其中 cys-149 几乎完全氧化的分子，以二硫化物二聚体或与谷胱甘肽混合的二硫化物形式存在，并且具有低于分子天然 (cys-149-SH) 形式活性的 1%。对大鼠 IL-10 进行定点诱变，用酪氨酸取代 cys-149，产生一种分子，该分子在溶解和重折叠时很容易采用活性构象，并且可以从细菌表达系统中以良好的产率回收。大鼠 IL-10tyr149 和商业大鼠 IL-10 制剂的生物活性比较证实，天然序列大鼠 IL-10 的活性降低或不存在。因此，建议生物合成类似物ratIL-10tyr149是研究IL-10在大鼠体内生物学作用的更有用的分子^[1]。

The ED₅₀ is <8 μg /mL as measured by C6 cells.

Rat

CHO

Tag Free

P29456 (S19-N178)

25325  [NCBI]

SKGHSIRGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNILLTDSLLQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVEQVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN

8-22 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Ball C, et al. Rat interleukin-10: production and characterisation of biologically active protein in a recombinantbacterial expression system. Eur Cytokine Netw. 2001 Mar;12(1):187-93.  [Content Brief]