IL-12 beta 蛋白, Mouse (HEK293, Fc)

IL-12 beta Protein, also known as natural killer cell stimulatory factor 2, is a common subunit (p40) of IL-12 and IL-23. IL-12 is a inflammatory factor expressed by activated macrophages, and involves in Th1-type immune response against cancer^[1]. IL-12 beta Protein located outside the cell membrane, involves in singalling mediated by Jak-STAT^[3]. IL-12 beta Protein consists of 335 amino acids (M1-S335) with a fibronectin type-III domain (233-324 a.a). IL-12 beta Protein, Mouse (M1-S335) is produced in HEK293 cells with a C-Terminal hFc-tag.

IL-12β蛋白，又称IL12 p40亚基或IL-12B，与IL-12 p35亚基（IL-12A）异二聚化形成IL-12，与IL23 p19亚基异二聚化形成IL-23，发挥不同的调节作用功能^[1]。
IL-12 和 IL23 属于 IL-12 家族，参与促炎反应，由活化的巨噬细胞表达，是 Th1 细胞发育的重要诱导剂^[2]。
IL-12 信号通过 p-STAT4 介导的 IL-12Rβ1 和 IL-12Rβ2 传递，而 IL-23 信号通过 p-STAT1 介导的 IL-12Rβ1 和 IL-23R 传递p-STAT3^[3]。
小鼠IL-12β蛋白氨基酸序列与人类差异较大（69.04%），但与大鼠相似度较高（92.24%） ).
白细胞介素 12 (IL-12) 家族已知是一种炎症因子，可诱导自身免疫性炎症，因此可能是自身免疫性炎症性疾病的原因，并且可能对肿瘤发生很重要^[4] .

Mouse IL-12 (mIL-12) has a significant stimulating effect on CTLL-2 cells and results 65-7% of CTLL2 cell population having mIL12 receptors^[5].
Mouse IL-12 (mIL-12) (.19-5 U/mL; 24-72 h) inhibits CTLL-2 cells proliferation without cell-lethal effect at 2 U/mL for 72 h^[5].

IL12b^-/- (p4-deficient) mice exhibits decreasing blood neutrophil numbers and an absence of serum levels of IL-17A^[6].
  IL12b^-/-/Itgb2^-/- (lacking adhesion molecules like beta2-integrins) mice has a reducing tissue mRNA expression of IL-17A compared with Itgb2^-/- controls and reduces total number of CD3+ IL-17A-producing Tn cells in the spleen and lamina propria^[6].
IL12b contributes to Aβ₄₂-induced neuronal damage and cognitive decline in APPPS1 Alzheimer's disease mouse model^[7].

Mouse

HEK293

C-hFc

P43432 (M23-S335)

16160  [NCBI]

Approximately 83 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.

• [1]. Lu X. Impact of IL-12 in Cancer. Curr Cancer Drug Targets. 2017;17(8):682-697.  [Content Brief]

  [2]. Gunsten S, et al. IL-12 p80-dependent macrophage recruitment primes the host for increased survival following a lethal respiratory viral infection. Immunology. 2009 Apr;126(4):500-13.  [Content Brief]

  [3]. Gotthardt D, Trifinopoulos J, Sexl V, Putz EM. JAK/STAT Cytokine Signaling at the Crossroad of NK Cell Development and Maturation. Front Immunol. 2019 Nov 12;10:2590.  [Content Brief]

  [4]. Tait Wojno ED, Hunter CA, Stumhofer JS. The Immunobiology of the Interleukin-12 Family: Room for Discovery. Immunity. 2019 Apr 16;50(4):851-870.  [Content Brief]

  [5]. Khatri A, et al. Murine CTLL-2 cells respond to mIL12: prospects for developing an alternative bioassay for measurement of murine cytokines IL12 and IL18. J Immunol Methods. 2007 Sep 30;326(1-2):41-53.  [Content Brief]

  [6]. Smith E, et al. IL-23 is required for neutrophil homeostasis in normal and neutrophilic mice. J Immunol. 2007 Dec 15;179(12):8274-9.  [Content Brief]

  [7]. Vom Berg J, et al. Inhibition of IL-12/IL-23 signaling reduces Alzheimer's disease-like pathology and cognitive decline. Nat Med. 2012 Dec;18(12):1812-9.  [Content Brief]