IL-12 蛋白, Human (Biotinylated, HEK293, His-Avi)

IL-35 protein plays a key role in immune regulation, forming IL-12 cytokine with IL12B or IL-35 cytokine with EBI3/IL27B. IL-12 modulates T cell and natural killer cell responses and induces interferon gamma production. IL-12 Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived IL-12 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. IL-12 Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of ~40-45 kDa.

IL-35 蛋白在免疫调节中发挥着关键作用，具有多功能性。它与 IL12B 异二聚化形成 IL-12 细胞因子，或与 EBI3/IL27B 异二聚化形成 IL-35 细胞因子。IL-12 主要由 B 细胞、树突状细胞、巨噬细胞和粒细胞等专业抗原呈递细胞产生，是先天抵抗和适应性免疫之间的重要纽带，调节 T 细胞和自然杀伤细胞反应，同时诱导干扰素-γ 的产生并有利于 T 辅助细胞 1 细胞的分化。从机制上讲，IL-12 通过由 IL12R1 和 IL12R2 亚基组成的受体发挥作用，导致细胞底物的酪氨酸磷酸化，并随后通过招募的磷酸化 STAT4 来调节细胞因子/生长因子响应基因。在 IL-35 的背景下，IL-35 对维持肝脏微环境中的免疫稳态有显着贡献，并作为免疫抑制细胞因子发挥作用。值得注意的是，IL-35 通过由 IL12RB2 和 gp130/IL6ST 异二聚体或同二聚体组成的非常规受体介导其作用，需要转录因子 STAT1 和 STAT4 进行信号传导。此外，IL-35 与 NBR1 相互作用，促进 IL-12 分泌。具有 EBI3/IL27B 的 IL-35 异二聚体（称为白细胞介素 IL-35）不是二硫键连接的，这与具有 IL12B 的二硫键连接的 IL-12 异二聚体不同。

Immobilized Anti-IL-12 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-12, His Tag with the EC₅₀ of <13.0ng/ml determined by ELISA.

Human

HEK293

C-Avi;C-His

P29459 (R23-S219) & P29460 (I23-S328)

3592  [NCBI]&3593  [NCBI]

Approximately 40-45 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5-8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.