IL-15 蛋白, Rhesus macaque (P.pastoris, His)

The IL-15 protein is a cytokine that plays a key role in inflammatory and protective immune responses by regulating innate and adaptive immune cells. It stimulates the proliferation of natural killer cells, T cells and B cells, and promotes the secretion of cytokines. IL-15 Protein, Rhesus macaque (P.pastoris, His) is the recombinant Rhesus Macaque-derived IL-15 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of IL-15 Protein, Rhesus macaque (P.pastoris, His) is 114 a.a., with molecular weight of ~16 kDa.

IL-15 蛋白是一种细胞因子，通过调节先天性和适应性免疫系统内的免疫细胞，在协调针对微生物入侵者和寄生虫的炎症和保护性免疫反应中发挥着关键作用。其影响范围包括刺激自然杀伤细胞、T 细胞和 B 细胞的增殖，同时还促进各种细胞因子的分泌。IL-15 通过诱导 IL8 和单核细胞趋化蛋白 1/CCL2 的产生，吸引中性粒细胞和单核细胞到达感染部位，从而在单核细胞中发挥作用。值得注意的是，IL-15 与大多数细胞因子不同，因为它与 IL15 产生细胞表面的高亲和力受体 IL15RA 相关表达。这种独特的表达模式使 IL-15 能够将信号传递至表达 IL2RB 和 IL2RG 受体亚基的靶细胞。与其受体结合后，IL-15 会触发 JAK1 和 JAK3 的磷酸化，招募并随后磷酸化转录 3/STAT3 和 STAT5 的信号转导器和激活剂。此外，在肥大细胞中，IL-15 诱导 STAT6 快速酪氨酸磷酸化，从而控制肥大细胞的存活和 IL4 等细胞因子的释放。

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is 2.330-2.534 ng/mL, corresponding to a specific activity is ≥3.946×10⁵ units/mg.

Rhesus Macaque

P. pastoris

N-6*His

P48092 (N49-S162)

699616  [NCBI]

NWVNVISDLKKIEDLIQSMHIDATLYTESDVHPSCKVTAMKCFLLELQVISHESGDTDIHDTVENLIILANNILSSNGNITESGCKECEELEEKNIKEFLQSFVHIVQMFINTS

Approximately 16 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.