IL-1RAcP/IL-1 R3 蛋白, Cynomolgus (HEK293, His)

IL-1RAcP is a co-receptor for IL1RL2, IL1R1 and IL1RL1 in the IL-36, IL-1 and IL-33 signaling system, respectively. IL-1RAcP is an essential component of the IL-1 receptor and plays an essential role in the inflammatory process^[1][2]. IL-1RAcP/IL-1 R3 Protein, Cynomolgus (HEK293, His) is a recombinant cynomolgus IL-1RAcP protein with a His tag at the C-terminus and is expressed in HEK293 cells.

白细胞介素1受体辅助蛋白（IL-1RAcP），也称为IL-1 R3，属于免疫球蛋白（Ig）超家族。IL-1RAcP在肝脏、皮肤、胎盘、胸腺和肺中均可检测到。
食蟹猴IL-1RAcP蛋白与人、大鼠、小鼠的氨基酸序列一致性为99.30%、88.60%、88.42%。
/> IL-1RAcP 包含三个 Ig 样结构域和一个名为 Toll/IL-1 受体 (TIR) 的胞质结构域（位置：403-546 aa），与信号转导有关。IL-1RAcP 还包含单个跨膜区。IL-1RAcP 分别是 IL-36、IL-1 和 IL-33 信号系统中 IL1RL2、IL1R1 和 IL1RL1 的共受体。IL-1RAcP 是 IL-1 受体信号转导复合物中不可或缺的分子，将质膜水平的事件与下游信号通路联系起来，介导 IL-1 依赖性转录因子激活和基因表达。
IL-1RAcP 发挥着重要作用在炎症过程中发挥重要作用，可用于肥胖、糖尿病、子宫内膜异位症、动脉粥样硬化等的研究^[1][2][3]。

Measured by its ability to inhibit IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 1.163 µg/mL in the presence of 1 μg/mL of recombinant human IL-1 R2 and 50 pg/mL rhIL-1 alpha, corresponding to a specific activity is 619.963 U/mg.

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  Measured by its ability to inhibit IL-1 alpha -induced IL-8 secretion in HepG2 human hepatocellular carcinoma cells. The ED₅₀ for this effect is 1.163 µg/mL in the presence of 1 μg/mL of recombinant human IL-1 R2 and 50 pg/mL rhIL-1 alpha, corresponding to a specific activity is 619.963 U/mg.

Cynomolgus

HEK293

C-His

P59822-1/NP_001028132.1 (S21-E359)

574387  [NCBI]

SERCDDWGLDTMRQIQVFEDEPARIKCPLFEHFLKFNYSTAHSAGLTLIWYWTRQDRDLEEPINFRLPENRISKEKDVLWFRPTLLNDTGNYTCMLRNTTYCSKVAFPLEVVQKDSCFNSPMKLPVHKLYIEYGIQRITCPNVDGYFPSSVKPTITWYMGCYKIQNFNNVIPEGMNLSFLIAFISNNGNYTCVVTYPENGRTFHLTRTLTVKVVGSPKNAVPPVIHSPNDHVVYEKEPGEELLIPCTVYFSFLMDSRNEVWWTIDGKKPDDIPIDVTINESISHSRTEDETRTQILSIKKVTSEDLKRSYVCHARSAKGEVAKAATVKQKVPAPRYTVE

Approximately 55-70 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Neveu PJ, et al. Mechanisms of behavioral and neuroendocrine effects of interleukin-1 in mice. Ann N Y Acad Sci. 2000;917:175-85.  [Content Brief]

  [2]. Zarezadeh Mehrabadi A, et al. The roles of interleukin-1 receptor accessory protein in certain inflammatory conditions. Immunology. 2022 May;166(1):38-46.  [Content Brief]

  [3]. Wesche H, et al. The interleukin-1 receptor accessory protein (IL-1RAcP) is essential for IL-1-induced activation of interleukin-1 receptor-associated kinase (IRAK) and stress-activated protein kinases (SAP kinases). J Biol Chem. 1997 Mar 21;272(12):7727-31.  [Content Brief]