IL-2 蛋白, Canine (C147S)

IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Canine (C147S) is the recombinant canine-derived IL-2 protein, expressed by E. coli , with tag free and C147S mutation.

IL-2 蛋白由激活的 CD4 阳性辅助 T 细胞以及较小程度的激活的 CD8 阳性 T 细胞和自然杀伤 (NK) 细胞产生，在免疫反应和耐受性中发挥着至关重要的作用。它与由高亲和力三聚体 IL-2R（IL2RA/CD25、IL2RB/CD122 和 IL2RG/CD132）或低亲和力二聚体 IL-2R（IL2RB 和 IL2RG）组成的受体复合物结合，导致下游信号传导通过 JAK1 和 JAK3 的磷酸化。这种激活会触发多种途径，包括 STAT、磷酸肌醇 3 激酶/PI3K 和丝裂原激活蛋白激酶/MAPK 途径。IL-2 蛋白作为 T 细胞生长因子，增强 NK 细胞的溶细胞活性，促进活化 B 细胞的增殖，并刺激免疫球蛋白的产生。它还通过控制调节性 T 细胞的存活和增殖来调节适应性免疫系统，这对于免疫耐受至关重要。此外，IL-2 蛋白参与效应 T 细胞亚群的分化和稳态，例如 Th1、Th2、Th17 和记忆 CD8 阳性 T 细胞。

1.Measured by its binding ability in a functional ELISA.Immobilized recombinant Canine IL2 at 10 μg/mL can bind recombinant human IL2Ra-Fc with a linear range of 0.3125-20 μg/mL.
2.Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED₅₀ for this effect is 0.3569 ng/mL, corresponding to a specific activity is 2.8×10⁶ units/mg.

Canine

E. coli

Tag Free

Q29416 (A21-T155, C147S)

403989  [NCBI]

APITSSSTKETEQQMEQLLLDLQLLLNGVNNYENPQLSRMLTFKFYTPKKATEFTHLQCLAEELKNLEEVLGLPQSKNVHLTDTKELISNMNVTLLKLKGSETSYNCEYDDETATITEFLNKWITFSQSIFSTLT

Approximately 15-16 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.