IL-2 蛋白, Mouse

IL-2 Protein, Mouse is an immunomodulatory cytokine with widely varying functions including promotion of apoptosis, proliferation and survival of lymphocytes.

白细胞介素-2 (IL-2) 是一种多方面的细胞因子，以根据细胞类型和状态促进增殖、存活和细胞死亡而闻名。例如，当抗原和 IL-2 丰富时，IL-2 仅在活化的 T 细胞中促进细胞死亡。IL-2 的可用性明显影响这一过程。IL-2 被硫酸乙酰肝素保留在血管中，并且具有生物活性的IL-2 被乙酰肝素酶从血管组织中释放出来。二聚体 IL-2 诱导的形态变化和快速细胞死亡表明细胞死亡是通过膜通透性破坏和随后的坏死介导的。IL-2 对免疫和非免疫系统中的细胞稳态机制具有直接且意想不到的广泛影响^[1]。

1.Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ this effect is < 0.2 ng/mL, corresponding to a specific activity is ≥ 5×10⁶ IU/mg.
2.Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×10⁶ units/mg.

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  Measured in a cell proliferation assay using CTLL-2 Cells. The ED₅₀ for this effect is 0.5117 ng/mL, corresponding to a specific activity is 1.954×10⁶ units/mg.

Mouse

E. coli

Tag Free

P04351 (A21-Q169)

16183  [NCBI]

APTSSSTSSSTAEAQQQQQQQQQQQQHLEQLLMDLQELLSRMENYRNLKLPRMLTFKFYLPKQATELKDLQCLEDELGPLRHVLDLTQSKSFQLEDAENFISNIRVTVVKLKGSDNTFECQFDDESATVVDFLRRWIAFCQSIISTSPQ

Approximately 15-20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Sodium Citrate, 0.2% Tween 80, pH 3.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wrenshall LE, et al. Identification of a cytotoxic form of dimeric interleukin-2 in murine tissues. PLoS One. 2014 Jul 14;9(7):e102191.  [Content Brief]