IL-22 蛋白, Human (His)

Animal-derived-free IL-22 protein is a cytokine that regulates tissue responses during inflammation and contributes to epithelial cell regeneration to maintain barrier function and prevent tissue damage. IL-22 Protein, Human (His) is the recombinant human-derived IL-22 protein, expressed by E. coli , with tag free.

IL-22 蛋白在调节炎症过程中的组织反应中发挥着关键作用，表明它在上皮细胞的再生中起着至关重要的作用，以保护损伤后的屏障功能并防止进一步的组织损伤。与大多数细胞因子不同，IL-22 对免疫细胞没有影响，它依赖于包含特定 IL22RA1 受体（在各种器官的非免疫细胞上发现）和共享亚基 IL10RB 的异二聚体受体。IL-22 与 IL22RA1 的结合激活酪氨酸激酶 JAK1 和 TYK2，随后触发 STAT3 激活。这反过来又通过 STAT3、ERK1/2 和 PI3K/AKT 途径促进细胞存活和增殖，从而促进 GSK3B 在“Ser-9”和 CTTN 处的磷酸化。此外，IL-22 促进上皮细胞扩散。

Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 128.1 pg/mL. Corresponding to a specific activity is 7.81×10⁶ U/mg.

Human

E. coli

N-6*His

Q9GZX6 (A34-I179)

50616  [NCBI]

APISSHCRLDKSNFQQPYITNRTFMLAKEASLADNNTDVRLIGEKLFHGVSMSERCYLMKQVLNFTLEEVLFPQSDRFQPYMQEVVPFLARLSNRLSTCHIEGDDLHIQRNVQKLKDTVKKLGESGEIKAIGELDLLFMSLRNACI

Approximately 17 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.