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  3. Interleukin & Receptors
  4. IL-23
  5. Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer 蛋白 (HEK293, His)

Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer 蛋白 (HEK293, His)

目录号: HY-P77703
COA 产品使用指南

IL-23 alpha 和IL-12 beta 分别是 IL-23p19 和 IL-12p40 亚基,通过异二聚的方式组成 IL-23。IL-23 与由 IL-12RB1 和 IL-23R 组成的异二聚受体结合,激活 Jak-Stat 信号级联,并优先作用于记忆 CD4(+) T 细胞。IL-23 还参与多个通路的激活,包括 p38 MAPKNF-κB,并促进促炎细胞因子如白细胞介素 IL-17A 的产生。重组食蟹猴 IL-23 alpha & 小鼠 IL-12 beta 异二聚体蛋白在 HEK293 细胞中产生,全长 481 个氨基酸,带有 N 端 His 标签。

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20 μg ¥2900 In-stock
50 μg ¥6400 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IL-23 alpha and IL-12 beta are the IL-23p19 and IL-12p40 subunits, respectively, composing IL-23 via heterodimerization manner. IL-23 binds to a heterodimeric receptor composed of IL-12RB1 and IL-23R, activates the Jak-Stat signaling cascade, acts on memory CD4(+) T cells preferentially[1]. IL-23 also involves in activation of several pathways including p38 MAPK or NF-κB and promotes the production of pro-inflammatory cytokines such as interleukin-17A/IL17A[2]. Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein is produced in HEK293 cells, with total length of 481 amino acids and a N-Terminal His-tag.

研究背景

IL-23 α 和 IL-12 β 分别也称为 IL23p19 和 IL12p40,以异二聚化方式组成 IL-23,发挥促炎作用并促进血管生成[1][5]
IL-23与IL-12 p35/p40、IL-27 EBI3/p28和IL-35 EBI3/p35同属于IL-12细胞因子家族,由多种免疫产生。[3]
IL-23 优先在记忆 CD4(+) T 细胞上表达,并激活 Jak -Stat信号级联。IL-23 会导致 IL-23 受体磷酸化,并形成对接位点来触发 STAT3 和 STAT4 的磷酸化信号[1]
IL-23 是维持 Th17 细胞活化的关键因素,通过结合 IL-23 受体产生 Th17 细胞因子,例如 IL17 A、IL-17 F 和 IL-22[2],从而产生细胞因子。
IL-23 还对自然杀伤细胞起作用,导致干扰素-γ分泌增加,增强抗体依赖性细胞毒性[4]
食蟹猴IL-23α蛋白氨基酸序列与人类高度相似(97.88%) )并且与小鼠(74.6%)和大鼠(79.37%)有很大不同
IL-23 在许多其他免疫病理学模型中促进炎症的发展,其中 IL-12 以前曾涉及这些模型,包括关节炎、肠道模型炎症和牛皮癣[6][7][8]

生物活性

Cynomolgus IL-23R, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-23 alpha&Mouse IL-12 beta, His Tag with an affinity constant of 2.36 nM as determined in SPR assay (Biacore T200).

种属

Cynomolgus

表达系统

HEK293

标签

N-His

蛋白编号

A0A2K5TLV4 (V22-P189)&P43432 (M23-S335)

基因 ID
蛋白结构
N-term
His
IL23A (V22-P189)
Accession # A0A2K5TLV4
C-term
N-term
IL12B (M23-S335)
Accession # P43432-1
C-term
中文名
重组狨猴 IL-23 alpha & 小鼠 IL-12 beta 异二聚体蛋白 (HEK293, His)
同用名
IL23 alpha; IL12 beta; IL23 alpha&IL12 beta
氨基酸序列

VPGGSSPAWAQCQQLSQKLCTLAWSAHPLVGHMDLREEGDEETTNDVPHIQCGDGCDPQGLRDNSQFCLQRIRQGLIFYEKLLGSDIFTGEPSLLPDSPVGQLHASLLGLSQLLQPEGHHWETQQIPSPSPSQPWQRLLLRFKILRSLQAFVAVAARVFAHGAATLSP&MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS

分子量

22 ( Cynomolgus IL23 alpha)&40-50 (Mouse IL12 beta) kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Cynomolgus IL-23 alpha & Mouse IL-12 beta Heterodimer Protein (HEK293, His)
目录号:
HY-P77703
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