IL-36 beta/IL-1F8 蛋白, Mouse

IL-36 beta (IL-1F8), a subform of IL-36 family, belongs to IL-1 superfamily. IL-36 beta mediates inflammatory response. L-36 beta binds to IL-36R and recruits the co-receptor IL-1RacP, and thereby activating NF-κB and MAPK signaling pathways, but the activation requires N-terminal cleavage by neutrophil granule-derived proteases^[1][2]. IL-36 beta/IL-1F8 Protein, Mouse is a recombinant mouse IL-36 beta without any tag, which is produced in E. coli.

IL-36β是IL-36家族的一个亚型，属于IL-1超家族。IL-36 beta 在单核细胞、T/B 淋巴细胞、骨髓、扁桃体、心脏、肺、睾丸、结肠、神经元细胞、神经胶质细胞中表达^[3]。
IL-36β 的氨基酸序列在不同物种中有所不同。小鼠 IL-36 beta 与人类的氨基酸序列同一性<40%。
L-36 beta 与 IL-36R 结合并招募辅助受体 IL-1RAcP。因此，异二聚体信号复合物使 2 条受体链的 Toll/IL-1R (TIR) 结构域非常接近，从而激活 NF-κB 和 MAPK 信号通路^[1]。但激活需要中性粒细胞颗粒衍生的蛋白酶（例如组织蛋白酶 G、弹性蛋白酶和蛋白酶-3^[2]）在 Arg5 的 N 端进行切割。IL-36β 在肠道炎症等炎症性疾病的发病机制中发挥重要作用^[4]。
IL-36β 是一种促炎因子。IL-36 beta 通过激活 NF-κB 和 MAPK 信号通路介导炎症反应^[2]。

IL-36 beta (mouse) (10 μg/mL, i.p., daily) exacerbates DSS-induce acute colitis mice^[4].
IL-36 beta (mouse) (1 μg per mouse) inhibits B16 tumor growth in C57/BL6 mice^[5].

Mouse

E. coli

Tag Free

Q9D6Z6 (S31-K183)

69677  [NCBI]

SSQSPRNYRVHDSQQMVWVLTGNTLTAVPASNNVKPVILSLIACRDTEFQDVKKGNLVFLGIKNRNLCFCCVEMEGKPTLQLKEVDIMNLYKERKAQKAFLFYHGIEGSTSVFQSVLYPGWFIATSSIERQTIILTHQRGKLVNTNFYIESEK

Approximately 16 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris,150 mM NaCl,1 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Bassoy EY, et al. Regulation and function of interleukin-36 cytokines. Immunol Rev. 2018 Jan;281(1):169-178.  [Content Brief]

  [2]. Zhou L,et al. Interleukin-36: Structure, Signaling and Function. Adv Exp Med Biol. 2021;21:191-210.  [Content Brief]

  [3]. Gresnigt MS, et al. Biology of IL-36 cytokines and their role in disease. Semin Immunol. 2013 Dec 15;25(6):458-65.  [Content Brief]

  [4]. Zhu J, et al. Interleukin-36β exacerbates DSS-induce acute colitis via inhibiting Foxp3+ regulatory T cell response and increasing Th2 cell response. Int Immunopharmacol. 2022 Jul;108:108762.  [Content Brief]

  [5]. Zhao X, et al. IL-36β Promotes CD8+ T Cell Activation and Antitumor Immune Responses by Activating mTORC1. Front Immunol. 2019 Aug 7;10:1803.  [Content Brief]