1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-5
  5. IL-5 蛋白, Human (HEK293, His-Avi)

IL-5 蛋白, Human (HEK293, His-Avi)

目录号: HY-P78467
产品使用指南

IL-5 蛋白主要由 T 淋巴细胞和 NK 细胞表达,对于嗜酸性粒细胞的存活、分化和趋化性至关重要。它还影响 B 细胞,诱导免疫球蛋白的产生、生长和分化。IL-5 蛋白激活 LYN、SYK 和 JAK2 等激酶,这些激酶通过 RAS-MAPK 和 JAK-STAT5 途径发出信号。它以二硫键连接的同二聚体形式存在,并与 IL5RA 和 CSF2RB 亚基相互作用。IL-5 蛋白, Human (HEK293, His-Avi) 是重组的 IL-5 蛋白,由 HEK293 表达,带有 N-His, N-Avi 标签。IL-5 蛋白, Human (HEK293, His-Avi) 全长 115 个氨基酸,分子量为 18-25 kDa。

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描述

The IL-5 Protein, predominantly expressed by T-lymphocytes and NK cells, is crucial for eosinophil survival, differentiation, and chemotaxis. It also affects B-cells, inducing immunoglobulin production, growth, and differentiation. IL-5 Protein activates kinases like LYN, SYK, and JAK2, which signal through RAS-MAPK and JAK-STAT5 pathways. It exists as a disulfide-linked homodimer and interacts with IL5RA and CSF2RB subunits. IL-5 Protein, Human (HEK293, His-Avi) is the recombinant human-derived IL-5 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of IL-5 Protein, Human (HEK293, His-Avi) is 115 a.a., with molecular weight of 18-25 kDa.

研究背景

IL-5蛋白是一种同型二聚体细胞因子,主要由T淋巴细胞和NK细胞表达,在嗜酸性粒细胞的存活、分化和趋化性中发挥着至关重要的作用。此外,IL-5 蛋白作用于激活的和静止的 B 细胞,诱导免疫球蛋白的产生、生长和分化。其生物学作用是通过由IL5RA亚基和细胞因子受体共同亚基β/CSF2RB组成的受体介导,导致LYN、SYK和JAK2等多种激酶的激活。这些激活的激酶通过 RAS-MAPK 和 JAK-STAT5 途径传播信号。IL-5 蛋白以二硫键连接的同二聚体形式存在,并与 IL5RA 和 CSF2RB 亚基相互作用。

种属

Human

表达系统

HEK293

标签

N-His;N-Avi

蛋白编号

P05113 (I20-S134)

基因 ID
蛋白结构
N-term
His-Avi
IL-5 (I20-S134)
Accession # P05113
C-term
同用名
Interleukin-5; IL-5; T-cell replacing factor; TRF; EDF
分子量

18-25 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

IL-5 Protein, Human (HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IL-5 Protein, Human (HEK293, His-Avi)
目录号:
HY-P78467
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