IL-5 蛋白, Mouse (CHO)

IL-5 Protein, Mouse (CHO) is a CHO cell derived hematopoietic growth factor expressed in Th2, mast cells and eosinophils.

白介素 5 (IL-5) 主要由 2 型 T 辅助淋巴细胞 (Th2) 产生，是一种嗜酸性粒细胞分化和激活因子。在动物模型中，正在探索 IL-5 活性的拮抗作用作为治疗多种与嗜酸性粒细胞相关疾病的潜在方法^[1]。白介素5（IL-5）以前被称为T细胞替代因子、B细胞生长和分化因子、集落形成单位生长刺激因子和嗜酸性粒细胞分化因子，主要由T细胞产生。小鼠 IL-5 作为小鼠 B 细胞的生长因子具有活性，但对人类 B 细胞没有活性。小鼠 IL-5 与细菌内毒素协同作用，刺激 B 细胞产生和分泌 IgM 和 IgA。IL-5 还被发现可以促进人和小鼠胸腺细胞产生细胞毒性细胞。IL-5 是早期造血祖细胞的有效生长促进剂。它在体外刺激小鼠、人和绵羊嗜酸性粒细胞的产生，并在体内控制嗜酸性粒细胞的产生。小鼠 IL-5 具有嗜酸性粒细胞增强活性^[2]。

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.1832-0.5 ng/mL, corresponding to a specific activity is 2×10⁶ - 5.46×10⁶ units/mg.

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  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 0.1832 ng/ml, corresponding to a specific activity is 5.46×10⁶ U/mg.

Mouse

CHO

Tag Free

P04401 (M21-G133)

16191  [NCBI]

MEIPMSTVVKETLTQLSAHRALLTSNETMRLPVPTHKNHQLCIGEIFQGLDILKNQTVRGGTVEMLFQNLSLIKKYIDRQKEKCGEERRRTRQFLDYLQEFLGVMSTEWAMEG

15-20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Yang S, et al. Canine interleukin-5: molecular characterization of the gene and expression of biologically active recombinant protein. J Interferon Cytokine Res. 2001 Jun;21(6):361-7.  [Content Brief]

  [2]. Seow HF, et al. Cloning and sequencing of an ovine interleukin-5 cDNA. DNA Seq. 1996;6(6):331-5.  [Content Brief]