Importin alpha-1/KPNA2 蛋白, Human (His)

Importin alpha-1/KPNA2 is essential for nuclear protein import, acting as an adapter for the nuclear receptor KPNB1. It directly binds the substrate to the nuclear localization signal to form a complex that docks with the nuclear pore complex mediated by KPNB1. Importin alpha-1/KPNA2 Protein, Human (His) is the recombinant human-derived Importin alpha-1/KPNA2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Importin alpha-1/KPNA2 Protein, Human (His) is 529 a.a., with molecular weight of ~60.0 kDa.

输入蛋白 alpha-1/KPNA2 作为核受体 KPNB1 的衔接蛋白，在核蛋白输入中发挥着关键作用。它表现出与含有简单或二分核定位信号 (NLS) 基序的底物的特异性和直接结合。输入蛋白/底物复合物通过 KPNB1 的介导与核孔复合物 (NPC) 对接，与核孔蛋白 FxFG 重复序列结合。随后，复合物通过需要能量、Ran 依赖性的机制通过孔进行移位。在 NPC 的核质侧，Ran 与 importin-beta 结合，导致三种成分的分离。然后输入蛋白-α和-β从细胞核重新输出到细胞质，其中GTP水解释放输入蛋白的Ran。核输入的方向性被认为是由细胞质和细胞核之间 GTP 和 GDP 结合形式的 Ran 的不对称分布决定的。Importin alpha-1 与 KPNB1 形成异二聚体，并与多种蛋白质相互作用，包括 ANP32E、CSE1L、RAN、PLAG1、APEX1、FRG1、ARL4A、CTNNBL1、NBN、SNAI1、SNAI2、BAG6、AIFM2 和 RSL1D1。

Human

E. coli

N-6*His

P52292 (M1-F529)

3838  [NCBI]

MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF

Approximately 60.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.