IRF5 蛋白, Human

The IRF5 protein is a key transcription factor in innate immunity and activates type I interferon (IFN) and inflammatory cytokine genes through endolysosomal Toll-like receptors (TLR7, TLR8, TLR9). It regulates IFN genes and IFN-stimulated genes (ISGs) by binding to ISREs in their promoters. IRF5 Protein, Human is the recombinant human-derived IRF5 protein, expressed by E. coli , with C-6*His labeled tag.

IRF5 是一种关键的转录因子，通过协调 I 型干扰素 (IFN) 基因（包括 IFNA 和 INFB）以及内溶酶体 Toll 样受体（TLR7、TLR8 和 TLR9）下游炎症细胞因子的激活，在先天免疫中发挥关键作用。。它通过与 I 型 IFN 基因和 IFN 刺激基因 (ISG) 启动子中的干扰素刺激反应元件 (ISRE) 结合，作为 I 型 IFN 基因和 IFN 刺激基因 (ISG) 转录的关键调节因子。值得注意的是，IRF5 有效激活 IFN-β (IFNB) 和 IFN-α (IFNA) 基因，通过 TLR 激活、MyD88 依赖性途径介导它们的诱导。IRF5 在 SARS-CoV-2 感染的背景下尤其重要，是 IFN 反应的重要转录调节因子。作为单体维持在自动抑制状态，其激活涉及由先天接头蛋白 TASL 促进的磷酸化，TASL 招募 IRF5，许可 IKBKB 对其进行磷酸化。后续步骤包括磷酸化 IRF5 从衔接蛋白上解离、其二聚化以及进入核以诱导 IFN。

Human

E. coli

C-6*His

Q13568-1 (M1-Q498)

3663  [NCBI]

MNQSIPVAPTPPRRVRLKPWLVAQVNSCQYPGLQWVNGEKKLFCIPWRHATRHGPSQDGDNTIFKAWAKETGKYTEGVDEADPAKWKANLRCALNKSRDFRLIYDGPRDMPPQPYKIYEVCSNGPAPTDSQPPEDYSFGAGEEEEEEEELQRMLPSLSLTEDVKWPPTLQPPTLRPPTLQPPTLQPPVVLGPPAPDPSPLAPPPGNPAGFRELLSEVLEPGPLPASLPPAGEQLLPDLLISPHMLPLTDLEIKFQYRGRPPRALTISNPHGCRLFYSQLEATQEQVELFGPISLEQVRFPSPEDIPSDKQRFYTNQLLDVLDRGLILQLQGQDLYAIRLCQCKVFWSGPCASAHDSCPNPIQREVKTKLFSLEHFLNELILFQKGQTNTPPPFEIFFCFGEEWPDRKPREKKLITVQVVPVAARLLLEMFSGELSWSADSIRLQISNPDLKDRMVEQFKELHHIWQSQQRLQPVAQAPPGAGLGVGQGPWPMHPAGMQ

Approximately 58.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 30% Glycerol, 1 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.