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  3. ISG15 E1/UBE1L 蛋白, Human (sf9, His, Strep)

ISG15 E1/UBE1L 蛋白, Human (sf9, His, Strep)

目录号: HY-P79454
产品使用指南

ISG15 E1/UBE1L 蛋白通过用 ATP 将泛素的 C 末端甘氨酸腺苷酸化,与其半胱氨酸残基形成硫酯键,从而启动泛素化。这会产生泛素-E1 硫酯和游离 AMP。ISG15 E1/UBE1L 蛋白, Human (sf9, His, Strep) 是重组的 ISG15 E1/UBE1L 蛋白,由 Sf9 insect cells 表达,带有 N-8*His, N-Strep 标签。ISG15 E1/UBE1L 蛋白, Human (sf9, His, Strep) 全长 1011 个氨基酸。

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描述

The ISG15 E1/UBE1L protein initiates ubiquitination by adenylating the C-terminal glycine of ubiquitin with ATP to form a thioester bond with its cysteine residue. This produces ubiquitin-E1 thioester and free AMP. ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is the recombinant human-derived ISG15 E1/UBE1L protein, expressed by Sf9 insect cells , with N-8*His, N-Strep labeled tag. The total length of ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) is 1011 a.a., .

研究背景

ISG15 E1/UBE1L 蛋白通过两步机制激活泛素来启动泛素化过程。它首先用 ATP 对泛素的 C 端甘氨酸残基进行腺苷酸化,随后在该甘氨酸残基和 E1 中的半胱氨酸残基之间形成硫酯键。这导致泛素-E1 硫酯和游离 AMP 的生成。除了在泛素激活中发挥作用外,ISG15 E1/UBE1L 还可催化甲型流感病毒 NS1 蛋白的 ISG 化,突出表明其参与抗病毒反应。该蛋白质积极参与蛋白质修饰过程,特别是蛋白质泛素化,强调了其通过翻译后修饰调节细胞途径的重要性。

种属

Human

表达系统

Sf9 insect cells

标签

N-8*His;N-Strep

蛋白编号

P41226 (D2-L1012)

基因 ID
蛋白结构
N-term
8*His-Strep
UBE1L (D2-L1012)
Accession # P41226
C-term
同用名
Ubiquitin-like modifier-activating enzyme 7; UBA7; Ubiquitin-activating enzyme 7; D8; Ubiquitin-activating enzyme E1 homolog; UBE1L; UBE2
纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ISG15 E1/UBE1L Protein, Human (sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ISG15 E1/UBE1L Protein, Human (sf9, His, Strep)
目录号:
HY-P79454
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