1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. JAM-A/CD321 Immunoglobulin-like Cell Adhesion Molecules
  5. Junctional Adhesion Molecule A (JAM-A)
  6. JAM-A/CD321 蛋白, Mouse (HEK293, Fc)

JAM-A/CD321 蛋白, Mouse (HEK293, Fc)

目录号: HY-P73855
产品使用指南

JAM-A/CD321 蛋白对于上皮紧密连接的形成至关重要,并且存在于原始细胞连接处,在那里它招募 PARD3。这种关联可能会阻碍 PARD3-JAM1 相互作用,从而阻碍紧密连接组装。JAM-A/CD321 蛋白, Mouse (HEK293, Fc) 是重组的 JAM-A/CD321 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

The JAM-A/CD321 protein is critical for the formation of epithelial tight junctions and is present at primitive cell junctions where it recruits PARD3.This association may hinder PARD3-JAM1 interaction and thus tight junction assembly.JAM-A/CD321 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived JAM-A/CD321 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

JAM-A/CD321 蛋白在上皮紧密连接的形成中起着至关重要的作用,并且存在于原始细胞连接的早期,在那里它招募 PARD3。PARD6-PARD3 复合物的结合可能阻碍 PARD3 和 JAM1 之间的相互作用,从而阻止紧密连接的组装。此外,JAM-A 参与调节单核细胞迁移,有助于上皮屏障的完整性。作为整合素 α-L/β-2 的配体,它参与记忆 T 细胞和中性粒细胞的迁移。此外,JAM-A 参与血小板活化并与 MPDZ 的第九个 PDZ 结构域相互作用。PARD3 和 PARD6B 之间的关联可能会破坏其与 PARD3 第一个 PDZ 结构域的相互作用。此外,JAM-A 与 ITGAL 相互作用(通过 I 结构域),进一步强调了它在细胞过程中的多方面参与。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

O88792 (K27-A242)

基因 ID
蛋白结构
N-term
JAM-A (K27-A242)
Accession # O88792
hFc
C-term
同用名
Junctional Adhesion Molecule A; JAM-A; JAM-1; PAM-1; CD321; F11R; JCAM
分子量

Approximately 57 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
JAM-A/CD321 Protein, Mouse (HEK293, Fc)
目录号:
HY-P73855
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