JAM-A/CD321 蛋白, Rat (HEK293, His)

The JAM-A/CD321 protein is critical for the formation of tight junctions in epithelial cells, participating in early junction development and recruiting PARD3. However, the formation of PARD6-PARD3 complex may hinder PARD3-JAM1 interaction, thus preventing tight junction assembly. JAM-A/CD321 Protein, Rat (HEK293, His) is the recombinant rat-derived JAM-A/CD321 protein, expressed by HEK293 , with C-His labeled tag. The total length of JAM-A/CD321 Protein, Rat (HEK293, His) is 212 a.a., with molecular weight of 25 & 28 kDa, respectively.

JAM-A/CD321 蛋白在上皮细胞紧密连接的形成中起着至关重要的作用。它参与细胞连接发育的早期阶段并招募 PARD3。然而，PARD6-PARD3复合物的形成可能会阻碍PARD3和JAM1之间的相互作用，从而导致紧密连接组装受到阻碍。此外，JAM-A/CD321 参与调节单核细胞的迁移，这对于维持上皮屏障的完整性至关重要。它还充当整合素 α-L/β-2 的配体，促进记忆 T 细胞和中性粒细胞的迁移。此外，JAM-A/CD321 与 MPDZ 的第九个 PDZ 结构域和 PARD3 的第一个 PDZ 结构域相互作用，PARD3 和 PARD6B 之间的关联可能会破坏这种相互作用。此外，它还与 ITGAL 相互作用（通过 I 域）。

Measured by the ability of the immobilized protein to inhibit the adhesion of Vitronectin on HUVEC cells. The ED₅₀ for this effect is 0.1574 μg/mL in the presence of 10 ng/mL Vitronectin. Corresponding to a specific activity is 6353.24 U/mg.

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  Measured by the ability of the immobilized protein to inhibit the adhesion of Vitronectin on HUVEC cells. The ED₅₀ for this effect is 0.1574 μg/mL in the presence of 10 ng/mL Vitronectin. Corresponding to a specific activity is 6353.24 U/mg.

Rat

HEK293

C-6*His

Q9JHY1 (K27-G238)

116479  [NCBI]

KGSVYSPQTAVQVPENDSVKLPCIYSGFSSPRVEWKFVQGSTTALVCYNNQITVPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEDGGQNYGEVSIHLTVLVPPSKPTVSIPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGVPMLTADAKKTRAFINSSYTIDPKSGDLVFDPVSAFDSGEYYCEAQNGYGTAMRSEAVRMEAVELNVGG

Approximately 25&28 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.