1. 重组蛋白
  2. CD Antigens Receptor Proteins
  3. Epithelial cell CD Proteins Endothelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. JAM-B/CD322 Immunoglobulin-like Cell Adhesion Molecules
  5. JAM-B/CD322
  6. JAM-B/CD322 蛋白, Rat (HEK293, Fc)

JAM-B/CD322 蛋白, Rat (HEK293, Fc)

目录号: HY-P73852
产品使用指南

连接粘附分子 2 (Jam2, JAM-b, CD322) 是免疫球蛋白超家族的成员,也是连接粘附分子 (JAM) 家族的成员。Jam2 是一种 I 型膜蛋白,定位于上皮细胞和内皮细胞的紧密连接处,具有整合素 α-4/β-1 结合活性,也是一种抑制性体树突信号。JAM-B/CD322 蛋白, Rat (HEK293, Fc) 是重组的 JAM-B/CD322 蛋白,由 HEK293 表达,带有 C-hFc 标签。JAM-B/CD322 蛋白, Rat (HEK293, Fc) 全长 208 个氨基酸,分子量约为 ~61 kDa。

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描述

Junctional adhesion molecule 2 (Jam2, Jam-B, CD322) is a member of the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. Jam2 is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. Jam2 has integrin alpha-4/beta-1 binding activity and an inhibitory somatodendritic cue. JAM-B/CD322 Protein, Rat (HEK293, Fc) is the recombinant rat-derived JAM-B/CD322 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of JAM-B/CD322 Protein, Rat (HEK293, Fc) is 208 a.a., with molecular weight of ~61 kDa.

研究背景

连接粘附分子 2 (Jam2, JAM-b, CD322) 是免疫球蛋白超家族的成员,也是连接粘附分子 (JAM) 家族的成员。Jam2 是一种 I 型膜蛋白,定位于上皮细胞和内皮细胞的紧密连接处。Jam2 作为一种粘附配体与多种免疫细胞类型相互作用,并可能在淋巴细胞归巢到次级淋巴器官中发挥作用。Jam2 还具有整合素 α-4/β-1 结合活性,并在许多细胞过程中发挥重要作用,如紧密连接组装、精子发生、细胞旁通透性调节、白细胞迁移、血管生成、肿瘤转移和细胞增殖。此外,Jam2 作为抑制性体树突信号,阻止神经元非轴突部分的髓鞘形成,有助于肌肉形成过程中的肌细胞融合[1][2][3]

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

Q3MHC0 (F29-N236)

基因 ID
蛋白结构
N-term
JAM-A (F29-N236)
Accession # Q3MHC0
hFc
C-term
同用名
C21orf43; CD322; JAM2; JAM-B; junctional adhesion molecule B; PRO245
分子量

Approximately 61 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
JAM-B/CD322 Protein, Rat (HEK293, Fc)
目录号:
HY-P73852
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