JAM-C/CD323 蛋白, Mouse (HEK293, His)

JAM-C/CD323 protein and JAM2 participate in a variety of cell-cell interactions and regulate cellular processes. It plays a key role in the homing and retention of hematopoietic cells in the bone marrow. JAM-C/CD323 Protein, Mouse (HEK293, His) is the recombinant mouse-derived JAM-C/CD323 protein, expressed by HEK293 , with C-His labeled tag.

JAM-C/CD323 蛋白作为连接粘附分子，与其受体 JAM2 参与异型细胞间相互作用，以调节不同的细胞过程。它在骨髓内造血干细胞和祖细胞的归巢和动员中发挥着关键作用，有助于它们保留在骨髓基质细胞的表面。此外，JAM-C 是白细胞外渗的核心，促进通过内皮细胞的迁移。在精子发生过程中，它在支持细胞和生殖细胞之间形成相互作用，这对于将生殖细胞锚定到支持细胞上并在精子细胞分化过程中建立细胞极性至关重要。JAM-C 作为 ITGAM 的反受体，介导白细胞-血小板相互作用并调节多形核中性粒细胞的跨上皮迁移。此外，它在血管生成、细胞迁移调节、肌生成过程中的肌细胞融合中发挥作用，并促进血管内皮细胞的趋化性，从而刺激血管生成。JAM-C 的多方面功能强调了它在各种细胞和生理过程中的重要性。

Measured by its ability to inhibit the adhesion of Jurkat cells on immobilized Human JAM-2. The ED₅₀ for this effect is 0.3033 μg/mL in the presence of 0.2 µg/mL Human JAM-2, corresponding to a specific activity is 3.297×10³ units/mg.

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  Measured by its ability to inhibit the adhesion of Jurkat cells on immobilized Human JAM-2. The ED₅₀ for this effect is 0.3033 μg/mL in the presence of 0.2 µg/mL Human JAM-2, corresponding to a specific activity is 3.297×10³ units/mg.

Mouse

HEK293

C-6*His

Q9D8B7/NP_075766.1 (E30-N241)

83964  [NCBI]

EAVNLKSSNRNPVVHEFESVELSCIITDSQTSDPRIEWKKIQDGQTTYVYFDNKIQGDLAGRTDVFGKTSLRIWNVTRSDSAIYRCEVVALNDRKEVDEITIELIVQVKPVTPVCRIPAAVPVGKTATLQCQESEGYPRPHYSWYRNDVPLPTDSRANPRFQNSSFHVNSETGTLVFNAVHKDDSGQYYCIASNDAGAARCEGQDMEVYDLN

Approximately 27-34 kDa due to the glycosylation

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.