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  4. Kallikrein-15 蛋白, Human (HEK293, Fc)

Kallikrein-15 蛋白, Human (HEK293, Fc)

目录号: HY-P77047
产品使用指南

Kallikrein-15 是一种激肽释放酶 (KLK),具有胰蛋白酶样活性,可能促进癌症转移。研究发现,Kallikrein-15 仅在成熟精子细胞中强烈表达,而在未成熟生殖细胞中不表达。KLK15 可能能够裂解许多细胞外基质 (ECM) 成分,与细胞外基质 (ECM) 受体基团相互作用,并与肿瘤发生相关。Kallikrein-15 蛋白, Human (HEK293, Fc) 是重组的 激肽释放酶 15 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

Kallikrein-15 is a kallikrein (KLK) with trypsin-like activity that may promote cancer metastasis. The study found that Kallikrein-15 is only strongly expressed in mature sperm cells and not in immature germ cells. KLK15 may be able to cleave many extracellular matrix (ECM) components, interact with the extracellular matrix (ECM) receptor group, and be associated with tumorigenesis. Kallikrein-15 Protein, Human (HEK293, Fc) is the recombinant human-derived Kallikrein-15 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

Kallikrein-15 (KLK15) 是一种激肽释放酶 (KLK)。先前的研究表明 KLK15 可能在前列腺癌中发挥作用。睾丸免疫组织学化学分析表明,KLK15 在成熟精子细胞中强烈表达,但在未成熟生殖细胞中不表达。KLK15 在前列腺上皮基底细胞层的亚细胞定位主要在细胞核中。KLK15 还具有胰蛋白酶样活性,并优先在精氨酸 (R) 之后裂解。KLK15 可能能够裂解许多细胞外基质 (ECM) 成分,其功能与某些 KLK 家族成员相似。KLK15 的酶活性受 pH、阳离子和丝氨酸蛋白酶抑制剂等不同因素的调节。值得注意的是,KLK15 最有可能通过与细胞外基质 (ECM) 受体基团相互作用与肿瘤发生相关,并可能通过这种机制促进转移。

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9H2R5/NP_059979.2 (Q17-N256)

基因 ID
蛋白结构
N-term
Kallikrein-15 (Q17-N256)
Accession # Q9H2R5/NP_059979.2
hFc
C-term
同用名
ACO protease; KLK15; kallikrein-related peptidase 15
分子量

Approximately 53.1 kDa.

纯度

Greater than 80% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Kallikrein-15 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Kallikrein-15 Protein, Human (HEK293, Fc)
目录号:
HY-P77047
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