Kirrel1/NEPH1 蛋白, Mouse (HEK293, His)

The Kirrel1/NEPH1 protein is essential for the normal function of the glomerular filtration barrier, helping to maintain a stable podocyte structure and interdigitated foot processes connected by specialized cell-to-cell junctions called slit septa. Additionally, it acts as a signaling protein that requires the presence of TEC kinase to fully transactivate the transcription factor AP-1. Kirrel1/NEPH1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Kirrel1/NEPH1 protein, expressed by HEK293 , with C-6*His labeled tag.

Kirrel1，也称为 NEPH1，对于维持肾小球滤过屏障的正常功能至关重要。它在保持稳定的足细胞结构中发挥着至关重要的作用，其特征是通过称为狭缝隔膜的特殊细胞与细胞连接点连接的指状足突。作为一种信号蛋白，Kirrel1 需要 TEC 激酶的存在才能完全反式激活转录因子 AP-1。它与 TJP1/ZO-1 和 NPHS2/podocin 等关键蛋白相互作用，有助于形成控制足细胞结构和功能的复杂分子网络。此外，Kirrel1 通过其 Ig 样结构域形成同二聚体，并且当酪氨酸磷酸化时，与信号传导接头蛋白 GRB2 相互作用。值得注意的是，它与 NPHS1/nephrin 的相互作用依赖于 Kirrel1 糖基化，凸显了其在足细胞生物学中调节作用的复杂性。

Mouse

HEK293

C-6*His

Q80W68-1 (L48-L525)

170643  [NCBI]

LPGTQTRFSQEPADQTVVAGQRAVLPCVLLNYSGIVQWTKDGLALGMGQGLKAWPRYRVVGSADAGQYNLEITDAELSDDASYECQATEAALRSRRAKLTVLIPPEETRIDGGPVILLQAGTPYNLTCRAFNAKPAATIIWFRDGTQQEGAVTSTELLKDGKRETTISQLLIEPTDLDIGRVFTCRSMNEAIPNGKETSIELDVHHPPTVTLSIEPQTVLEGERVIFTCQATANPEILGYRWAKGGFLIEDAHESRYETNVDYSFFTEPVSCEVYNKVGSTNVSTLVNVHFAPRIVVYPKPTTTDIGSDVTLTCVWVGNPPLTLTWTKKDSNMVLSNSNQLLLKSVTQADAGTYTCRAIVPRIGVAEREVPLYVNGPPIISSEAVQFAVRGDGGKVECFIGSTPPPDRIAWAWKENFLEVGTLERYTVERTNSGSGVLSTLTINNVMEADFQTHYNCTAWNSFGPGTAIIQLEEREVL

60-90 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.