KRAS 蛋白, Human (G12S, His)

KRAS protein is a key Ras family member that binds GDP/GTP and has intrinsic GTPase activity. Its critical role in regulating cell proliferation emphasizes its importance in cellular processes. KRAS Protein, Human (G12S, His) is the recombinant human-derived KRAS, expressed by E. coli , with N-6*His labeled tag. ,

KRAS 是 Ras 蛋白家族的关键成员，具有结合 GDP/GTP 的能力，并具有内在的 GTP 酶活性。它对细胞增殖调节的重要参与强调了它在细胞过程中的重要性。值得注意的是，KRAS 在促进致癌事件中发挥着重要作用，特别是在结直肠癌 (CRC) 中，它以 ZNF304 依赖性方式诱导肿瘤抑制基因 (TSG) 的转录沉默。这种多方面的功能凸显了 KRAS 作为细胞动力学的关键参与者，并强调了它在正常和病理细胞过程中的相关性。

1.Biological activity has been tested in vitro. 2.Measured by its ability to catalyze 2m substrate GTP. which can be measured by absorbance at 620 nm that incubate at room temperature for 10 minutes. The specific activity is 716.25 nmo/min/mg.

Human

E. coli

N-6*His

AAH13572.1 (T2-C185, G12S)

3845  [NCBI]

TEYKLVVVGASGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEKMSKDGKKKKKKSKTKC

Approximately 25-30 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 7.4, 5% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.