L-Selectin/CD62L 蛋白, Rat (HEK293, His)

L-selectin/CD62L is a calcium-dependent lectin that binds adjacent glycoproteins to allow lymphocyte adhesion to endothelial cells in peripheral lymph nodes. This encourages leukocytes to bind and roll within endothelial tissue. L-Selectin/CD62L Protein, Rat (HEK293, His) is the recombinant rat-derived L-Selectin/CD62L protein, expressed by HEK293 , with C-His labeled tag.

L-选择素/CD62L 是一种钙依赖性凝集素，通过与相邻细胞上的糖蛋白结合，在细胞粘附中发挥关键作用。它促进淋巴细胞粘附到周围淋巴结高内皮微静脉的内皮细胞上，促进白细胞在内皮组织内的初始束缚和滚动。白细胞的募集和滚动取决于与 SELPLG/PSGL1 和 PODXL2 的相互作用，其中 SELPLG 和 PODXL2 的唾液酸 Lewis X 聚糖修饰以及 SELPLG 的酪氨酸硫酸化修饰至关重要。值得注意的是，SELPLG 的“Tyr-51”上的硫酸化成为 L-选择素结合的关键因素，强调了其在促进细胞相互作用和粘附过程中的重要性。

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5×10⁴ cells/well are added to Rat L-Selectin coated plates (10 μg/mL, 100 μL/well), will induce 52.78% adhesion on U937 cells after 1 hour at 37°C.

Rat

HEK293

C-His

P30836 (W39-N332)

29259  [NCBI]

WTYHYSERSMNWENARKFCKHNYTDLVAIQNKREIEYLEKTLPKNPTYYWIGIRKIGKTWTWVGTNKTLTKEAENWGTGEPNNKKSKEDCVEIYIKRERDSGKWNDDACHKRKAALCYTASCQPESCNRHGECVETINNNTCICDPGYYGPQCQYVIQCEPLKAPELGTMNCIHPLGDFSFQSQCAFNCSEGSELLGNAKTECGASGNWTYLEPICQVIQCMPLAAPDLGTMECSHPLANFSFTSACTFTCSEETDLIGERKTVCRSSGSWSSPSPICQKTKRSFSKIKEGDYN

Approximately 43-55 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.